Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets

• Published in: Viruses Vol. 10; no. 3; p. 106
• Main Authors: Hou, Xingyu, Jiang, Xinpeng, Jiang, Yanping, Tang, Lijie, Xu, Yigang, Qiao, Xinyuan, Min, Liu, Wen, Cui, Ma, Guangpeng, Li, Yijing
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.03.2018; MDPI
• Subjects: dendritic cell-targeting peptide; Dendritic cells; Diarrhea; Enzyme-linked immunosorbent assay; Epidemics; Genetic engineering; Helper cells; Immune response; Immunoglobulin A; Immunoglobulin G; Infections; Inflammation; Lactobacillus; Lactobacillus casei; Lactobacillus casei vaccine; Lymph nodes; Lymphocytes T; Mucosal immunity; Phenotypes; porcine epidemic diarrhea virus; Rodents; T cell receptors; T-helper cell; Transforming growth factor; Transforming growth factor-b; Transmissible gastroenteritis; Vaccination; Vaccines; dendritic cell-targeting peptide; porcine epidemic diarrhea virus; Lactobacillus casei vaccine; T-helper cell; mucosal immunity
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v10030106

Porcine epidemic diarrhea (PED) is a highly contagious disease in newborn piglets. In our previous study, a genetically engineered oral vaccine ( ) expressing a dendritic cell (DC)-targeting peptide fused with porcine epidemic diarrhea virus (PEDV) COE antigen was developed. This vaccine induced significant levels of anti-PEDV specific IgG and IgA antibody responses in mice, indicating a potential strategy against PEDV infection. In this study, was used for oral vaccination of newborn piglets against PEDV. We then assessed the immune responses and protection efficacy of . An indirect enzyme-linked immunosorbent assay (ELISA) showed that the recombinant vaccine elicits a specific systemic and mucosal immune response. The T-helper cells mediated by and PEDV infection display a Th1 phenotype. The histopathological results showed that promotes lymphocyte proliferation and effectively protects piglets against PEDV infection. The transforming growth factor-β level indicated that the recombinant vaccine plays a role in anti-inflammatory responses in mesenteric lymph nodes during PEDV infection. These results show that is a potential vaccine against PEDV infection.