Metabolic signature of Mycobacterium avium subsp. paratuberculosis infected and infectious dairy cattle by integrating nuclear magnetic resonance analysis and blood indices

• Published in: Frontiers in veterinary science Vol. 10; p. 1146626
• Main Authors: Massaro, Andrea, Tata, Alessandra, Pallante, Ivana, Bertazzo, Valentina, Bottazzari, Massimo, Paganini, Laura, Dall'Ava, Brunella, Stefani, Annalisa, De Buck, Jeroen, Piro, Roberto, Pozzato, Nicola
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 17.04.2023
• Subjects: biomarker; LASSO; metabolomics; NMR; paratuberculosis; prediction model; Veterinary Science; paratuberculosis; NMR; Mycobacterium avium subsp. paratuberculosis; prediction model; metabolomics; LASSO; biomarker; pathway analysis
• ISSN: 2297-1769; 2297-1769
• DOI: 10.3389/fvets.2023.1146626

The early diagnosis of subsp. (MAP) is one of the current challenges of farmers and veterinarians. This work aimed to investigate the changes in metabolic levels associated with natural MAP infection in infected and infectious dairy cattle. The study included sera from 23 infectious/seropositive, 10 infected but non-infectious/seronegative, and 26 negative Holstein Fresian cattle. The samples were selected from a collection of samples gathered during a prospective study. The samples were analyzed by quantitative nuclear magnetic resonance (NMR) spectroscopy and routine blood chemistry. The blood indices and the H NMR data were concatenated by low-level data fusion, resulting in a unique global fingerprint. Afterwards, the merged dataset was statistically analyzed by the least absolute shrinkage and selection operator (LASSO), which is a shrinkage and selection method for supervised learning. Finally, pathways analysis was performed to get more insights on the possible dysregulated metabolic pathways. The LASSO model achieved, in a 10 time repeated 5-fold cross-validation, an overall accuracy of 91.5% with high values of sensitivity and specificity in classifying correctly the negative, infected, and infectious animals. The pathway analysis revealed MAP-infected cattle have increased tyrosine metabolism and enhanced phenylalanine, tyrosine and tryptophan biosynthesis. The enhanced synthesis and degradation of ketone bodies was observed both in infected and infectious cattle. In conclusion, fusing data from multiple sources has proved to be useful in exploring the altered metabolic pathways in MAP infection and potentially diagnosing negative animals within paratuberculosis-infected herds.