Survival of probiotic bacteria in a whey cheese vector submitted to environmental conditions prevailing in the gastrointestinal tract

• Published in: International dairy journal Vol. 15; no. 6; pp. 921 - 927
• Main Authors: Madureira, A.R., Pereira, C.I., Truszkowska, K., Gomes, A.M., Pintado, M.E., Malcata, F.X.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01.06.2005; Elsevier Science
• Subjects: Bifidobacteria; Bifidobacterium animalis; Bile salts; Biological and medical sciences; Dairy products; digestive tract; Food industries; food intake; food microbiology; Fundamental and applied biological sciences. Psychology; Gastric acid; hydrochloric acid; Lactobacilli; Lactobacillus acidophilus; Lactobacillus paracasei subsp. paracasei; Milk and cheese industries. Ice creams; pepsin; physical models; probiotics; simulation models; viability; whey cheeses; Dairy products; Bifidobacteria; Gastric acid; Lactobacilli; Bile salts; Probiotic; Lactic acid bacteria; Digestive system; Bile salt; Dairy product; Cheese; Gastrointestinal; Whey; Survival; Dairy industry; Bacteria; Lactobacillaceae; Bile salts: Dairy products; Vector; Lactobacillus
• ISSN: 0958-6946; 1879-0143
• DOI: 10.1016/j.idairyj.2004.08.025

Various foods may be used to deliver probiotic bacteria into the gastrointestinal tract; one such example is Requeijão, a Portuguese whey cheese. Survival and stability of Bifidobacterium animalis strains BLC-1, Bb-12, and Bo, Lactobacillus acidophilus strains LAC-1 and Ki, L. paracasei ssp. paracasei strain LCS-1 and L. brevis strain LMG 6906 inoculated into Requeijão, when exposed to simulated gastrointestinal tract conditions, were assessed. Homogenates of inoculated whey cheese in 0.85% (w/v) sterile saline water were exposed to a solution of hydrochloric acid (pH 2.5–3.0) and pepsin (1000 units mL –1) at 37 °C, and then to 0.3% (w/v) bile salts after 60 or 120 min of acid exposure. All bacterial strains retained their initial viable cell numbers. Bifidobacterium animalis strains Bb-12 and Bo, and L. brevis strain LMG 6906 exhibited the highest viable cell numbers when exposed to bile salts, whereas the other strains had variable death rates.