Drosophila as a model host for Pseudomonas aeruginosa infection

• Published in: Journal of bacteriology Vol. 183; no. 4; pp. 1466 - 1471
• Main Authors: D'Argenio, D A, Gallagher, L A, Berg, C A, Manoil, C
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.02.2001
• Series: Note
• Subjects: Anatomy & physiology; Animals; Bacteria; Bacteriology; chpA gene; chpB gene; chpC gene; chpD gene; chpE genes; Disease Models, Animal; Drosophila melanogaster; Drosophila melanogaster - immunology; Drosophila melanogaster - microbiology; Female; Genes, Bacterial; Insects; Movement; Multigene Family; Mutation; Myxococcus xanthus - pathogenicity; Physiology and Metabolism; pilG gene; pilH gene; pilI gene; pilJ gene; pilL genes; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - pathogenicity; Pseudomonas Infections; Selection, Genetic; virulence factors
• ISSN: 0021-9193; 1098-5530
• DOI: 10.1128/JB.183.4.1466-1471.2001

Using the fruit fly Drosophila melanogaster as model host, we have identified mutants of the bacterium Pseudomonas aeruginosa with reduced virulence. Strikingly, all strains strongly impaired in fly killing also lacked twitching motility; most such strains had a mutation in pilGHIJKL chpABCDE, a gene cluster known to be required for twitching motility and potentially encoding a signal transduction system. The pil chp genes appear to control the expression of additional virulence factors, however, since the wild-type fly-killing phenotype of a subset of mutants isolated on the basis of their compact colony morphology indicated that twitching motility itself was not required for full virulence in the fly.