Zinc-modified titanium surface enhances osteoblast differentiation of dental pulp stem cells in vitro

Zinc is an essential trace element that plays an important role in differentiation of osteoblasts and bone modeling. This in vitro study aimed to evaluate the osteoblast differentiation of human dental pulp stem cells (DPSCs) on zinc-modified titanium (Zn-Ti) that releases zinc ions from its surface...

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Bibliographic Details
Published inScientific reports Vol. 6; no. 1; p. 29462
Main Authors Yusa, Kazuyuki, Yamamoto, Osamu, Takano, Hiroshi, Fukuda, Masayuki, Iino, Mitsuyoshi
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 08.07.2016
Subjects
Alkaline Phosphatase - genetics
Alkaline Phosphatase - metabolism
Biomarkers - metabolism
Bone marrow
Cell Differentiation - drug effects
Cell Proliferation - drug effects
Cells, Cultured
Dental pulp
Dental Pulp - cytology
Dental Pulp - drug effects
Dental Pulp - metabolism
Dentistry
Extracellular Matrix - drug effects
Gene expression
Growth factors
Humans
In Vitro Techniques
Mineralization
Osteoblasts - cytology
Osteoblasts - drug effects
Phosphatase
Shear strength
Stem cells
Stem Cells - cytology
Stem Cells - drug effects
Stem Cells - metabolism
Surgery
Titanium
Titanium - chemistry
Transplants & implants
Up-Regulation
Zinc - chemistry
Zinc - pharmacology
Japan
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Summary:Zinc is an essential trace element that plays an important role in differentiation of osteoblasts and bone modeling. This in vitro study aimed to evaluate the osteoblast differentiation of human dental pulp stem cells (DPSCs) on zinc-modified titanium (Zn-Ti) that releases zinc ions from its surface. Based on real-time PCR, alkaline phosphatase (ALP) activity and Western blot analysis data, we investigated osteoblast differentiation of DPSCs cultured on Zn-Ti and controls. DPSCs cultured on Zn-Ti exhibited significantly up-regulated gene expression levels of osteoblast-related genes of type I collagen (Col I), bone morphogenetic protein 2 (BMP2), ALP, runt-related transcription factor 2 (Runx2), osteopontin (OPN), and vascular endothelial growth factor A (VEGF A), as compared with controls. We also investigated extracellular matrix (ECM) mineralization by Alizarin Red S (ARS) staining and found that Zn-Ti significantly promoted ECM mineralization when compared with controls. These findings suggest that the combination of Zn-Ti and DPSCs provides a novel approach for bone regeneration therapy.
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ISSN:2045-2322
2045-2322
DOI:10.1038/srep29462