Escherichia coli O157:H7 tir 255 T > A allele strains differ in chromosomal and plasmid composition

• Published in: Frontiers in microbiology Vol. 14; p. 1303387
• Main Authors: Weinroth, Margaret D, Clawson, Michael L, Harhay, Gregory P, Eppinger, Mark, Harhay, Dayna M, Smith, Timothy P L, Bono, James L
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 15.12.2023
• Subjects: bacterial genomics; Bacteriophage; comparative genomics; Microbiology; Plasmids; STEC; whole genome sequencing (WGS); bacterial genomics; Plasmids; STEC; Bacteriophage; Translocated Intimin Receptor; comparative genomics; whole genome sequencing (WGS)
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2023.1303387

Shiga toxin-producing (STEC) O157:H7 strains with the T allele in the translocated intimin receptor polymorphism ( ) 255 A > T gene associate with human disease more than strains with an A allele; however, the allele is not thought to be the direct cause of this difference. We sequenced a diverse set of STEC O157:H7 strains (26% A allele, 74% T allele) to identify linked differences that might underlie disease association. The average chromosome and pO157 plasmid size and gene content were significantly greater within the 255 A allele strains. Eighteen coding sequences were unique to 255 A allele chromosomes, and three were unique to 255 T allele chromosomes. There also were non-pO157 plasmids that were unique to each 255 allele variant. The overall average number of prophages did not differ between 255 allele strains; however, there were different types between the strains. Genomic and mobile element variation linked to the 255 polymorphism may account for the increased frequency of the T allele isolates in human disease.