Chronic alcohol administration alters metabolomic profile of murine bone marrow

People with hazardous alcohol use are more susceptible to viral, bacterial, and fungal infections due to the effect of alcohol on immune system cell function. Metabolized ethanol reduces NAD to NADH, affecting critical metabolic pathways. Here, our aim was to investigate whether alcohol is metaboliz...

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Published inFrontiers in immunology Vol. 14; p. 1128352
Main Authors Pontes Pereira, Tássia Tatiane, Fideles Duarte-Andrade, Filipe, Gardone Vitório, Jéssica, do Espírito Santo Pereira, Taiane, Braga Martins, Flavia Rayssa, Marques Souza, Jéssica Amanda, Malacco, Nathália Luisa, Mathias Melo, Eliza, Costa Picossi, Carolina Raíssa, Pinto, Ernani, Santiago Gomez, Ricardo, Martins Teixeira, Mauro, Nori de Macedo, Adriana, André Baptista Canuto, Gisele, Soriani, Frederico Marianetti
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 05.04.2023
Subjects
alcoholism
Animals
Bone Marrow
cell function
Ethanol - pharmacology
immune system
Immunology
metabolites
Metabolome
Metabolomics - methods
Mice
Mice, Inbred C57BL
cell function
immune system
alcoholism
metabolites
bone marrow
metabolome
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Summary:People with hazardous alcohol use are more susceptible to viral, bacterial, and fungal infections due to the effect of alcohol on immune system cell function. Metabolized ethanol reduces NAD to NADH, affecting critical metabolic pathways. Here, our aim was to investigate whether alcohol is metabolized by bone marrow cells and if it impacts the metabolic pathways of leukocyte progenitor cells. This is said to lead to a qualitative and quantitative alteration of key metabolites which may be related to the immune response. We addressed this aim by using C57BL/6 mice under chronic ethanol administration and evaluating the metabolomic profile of bone marrow total cells by gas chromatography-coupled mass spectrometry (GC-MS). We identified 19 metabolites. Our data demonstrated that chronic ethanol administration alters the metabolomic profile in the bone marrow, resulting in a statistically diminished abundance of five metabolites in ethanol-treated animals: uracil, succinate, proline, nicotinamide, and tyrosine. Our results demonstrate for the first time in the literature the effects of alcohol consumption on the metabolome content of hematopoietic tissue and open a wide range of further studies to investigate mechanisms by which alcohol compromises the cellular function of the immune system.
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Edited by: Samantha Yeligar, Emory University, United States
Reviewed by: Liz Simon, Louisiana State University, United States; Rachel McMahan, University of Colorado Anschutz Medical Campus, United States
This article was submitted to Nutritional Immunology, a section of the journal Frontiers in Immunology
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2023.1128352