Application of single-cell sequencing to the research of tumor microenvironment

• Published in: Frontiers in immunology Vol. 14; p. 1285540
• Main Authors: Chen, Sijie, Zhou, Zhiqing, Li, Yu, Du, Yuhui, Chen, Guoan
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 27.10.2023
• Subjects: Cell Differentiation; clinical applications; Epigenome; Humans; immunological therapy; Immunology; Neoplasms - genetics; precision medicine; single-cell sequencing; Transcriptome; tumor microenvironment; Tumor Microenvironment - genetics; single-cell sequencing; precision medicine; tumor microenvironment; clinical applications; immunological therapy
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2023.1285540

Single-cell sequencing is a technique for detecting and analyzing genomes, transcriptomes, and epigenomes at the single-cell level, which can detect cellular heterogeneity lost in conventional sequencing hybrid samples, and it has revolutionized our understanding of the genetic heterogeneity and complexity of tumor progression. Moreover, the tumor microenvironment (TME) plays a crucial role in the formation, development and response to treatment of tumors. The application of single-cell sequencing has ushered in a new age for the TME analysis, revealing not only the blueprint of the pan-cancer immune microenvironment, but also the heterogeneity and differentiation routes of immune cells, as well as predicting tumor prognosis. Thus, the combination of single-cell sequencing and the TME analysis provides a unique opportunity to unravel the molecular mechanisms underlying tumor development and progression. In this review, we summarize the recent advances in single-cell sequencing and the TME analysis, highlighting their potential applications in cancer research and clinical translation.