Synthesis and immunologic characterisation of Mycobacterium tuberculosis lipoarabinomannan specific oligosaccharide–protein conjugates

• Published in: Vaccine Vol. 17; no. 22; pp. 2853 - 2861
• Main Authors: Hamasur, Beston, Källenius, Gunilla, Svenson, Stefan B
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 16.07.1999; Elsevier
• Subjects: Animals; Antibodies, Bacterial - biosynthesis; Antigens, Bacterial - chemistry; Antigens, Bacterial - immunology; Bacterial Proteins - administration & dosage; Bacterial Proteins - chemical synthesis; Bacterial Proteins - immunology; Bacteriology; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; Immunoglobulin G - biosynthesis; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - chemical synthesis; Lipopolysaccharides - immunology; Medicin och hälsovetenskap; Microbiology; Mycobacterium tuberculosis; Mycobacterium tuberculosis - chemistry; Mycobacterium tuberculosis - immunology; Oligosaccharides - administration & dosage; Oligosaccharides - chemical synthesis; Oligosaccharides - immunology; Rabbits; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Conjugate - administration & dosage; Vaccines, Conjugate - chemistry; Toxoid; Rabbit; Lagomorpha; Property formulation relationship; Glycolipid; Vertebrata; Mammalia; Immunogenicity; Mycobacterium tuberculosis; Mycobacteriales; Mycobacteriaceae; Acellular vaccine; Bacteria; Actinomycetes; Conjugated compound; Humoral immunity; Carrier protein
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(99)00124-3

Lipoarabinomannan (LAM) is a major structural surface component of all mycobacteria, and has been reported to have a wide range of biological effects. Immunogenic LAM specific oligosaccharide protein conjugates were synthesized and immunologically characterized. Oligosaccharides were derived from LAM purified from Mycobacterium tuberculosis H37 Rv and covalently conjugated to tetanus toxoid and cross reactive mutant (CRM197) diphtheria toxoid. Both types of LAM oligosaccharide protein conjugates proved to be highly immunogenic, inducing a boosterable T helper cell dependent IgG response. These conjugates are currently evaluated as components in a subcellular experimental TB vaccine.