The role of the gut-microbiome-brain axis in metabolic remodeling amongst children with cerebral palsy and epilepsy

• Published in: Frontiers in neurology Vol. 14; p. 1109469
• Main Authors: Peng, Ye, Chiu, Annie T. G., Li, Vivien W. Y., Zhang, Xi, Yeung, Wai L., Chan, Sophelia H. S., Tun, Hein M.
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 27.02.2023
• Subjects: cerebral palsy; drug resistant epilepsy; epilepsy; gut metabolome; gut microbiome; gut-brain axis; Neurology; epilepsy; gut metabolome; gut microbiome; cerebral palsy; multi-omics; drug resistant epilepsy; gut-brain axis
• ISSN: 1664-2295; 1664-2295
• DOI: 10.3389/fneur.2023.1109469

Epilepsy-associated dysbiosis in gut microbiota has been previously described, but the mechanistic roles of the gut microbiome in epileptogenesis among children with cerebral palsy (CP) have yet to be illustrated. Using shotgun metagenomic sequencing coupled with untargeted metabolomics analysis, this observational study compared the gut microbiome and metabolome of eight children with non-epileptic cerebral palsy (NECP) to those of 13 children with cerebral palsy with epilepsy (CPE). Among children with CPE, 8 had drug-sensitive epilepsy (DSE) and five had drug-resistant epilepsy (DRE). Characteristics at enrollment, medication history, and 7-day dietary intake were compared between groups. At the species level, CPE subjects had significantly lower abundances of and but higher abundances of and . By contrast, DRE subjects had a significantly higher colonization of . Regarding microbial functional pathways, CPE subjects had decreased abundances of pathways for serine degradation, quinolinic acid degradation, glutamate degradation I, glycerol degradation, sulfate reduction, and nitrate reduction but increased abundances of pathways related to ethanol production. As for metabolites, CPE subjects had higher concentrations of kynurenic acid, 2-oxindole, dopamine, 2-hydroxyphenyalanine, 3,4-dihydroxyphenylglycol, L-tartaric acid, and D-saccharic acid; DRE subjects had increased concentrations of indole and homovanilic acid. In this study, we found evidence of gut dysbiosis amongst children with cerebral palsy and epilepsy in terms of gut microbiota species, functional pathways, and metabolites. The combined metagenomic and metabolomic analyses have shed insights on the potential roles of and in neuroprotection. The combined analyses have also provided evidence for the involvement of GMBA in the epilepsy-related dysbiosis of kynurenine, serotonin, and dopamine pathways and their complex interplay with neuroimmune and neuroendocrinological pathways.