Attenuation of quorum sensing system and virulence in Vibrio cholerae by phytomolecules
The a gram-negative bacterium, is the causative agent of cholera. Quorum sensing is a cell-to-cell communication that leads to gene expression, accumulation of signaling molecules, biofilm formation, and production of virulence factors. The quorum sensing pathway in is regulated by O, and biofilm fo...
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Published in | Frontiers in microbiology Vol. 14; p. 1133569 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
30.03.2023
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Subjects | |
Online Access | Get full text |
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Summary: | The
a gram-negative bacterium, is the causative agent of cholera. Quorum sensing is a cell-to-cell communication that leads to gene expression, accumulation of signaling molecules, biofilm formation, and production of virulence factors. The quorum sensing pathway in
is regulated by
O, and biofilm formation and other virulence factors are positively controlled by
A and negatively by
R. Hence, targeting the global regulator
O would be a promising approach to modulate the QS to curtail
pathogenesis. The present study investigated the modulating activity of quercetin and naringenin on biofilm formation and quorum-sensing regulated phenotypes in
. Then after we determined the anti-quorum sensing capability of phytomolecules against the model organism
. Also, we performed flow cytometry for live/dead bacteria, MTT assay, CLSM, and growth curve analysis to determine their role as QS modulators rather than anti-bacterial.
strains VC287 and N16961 formed thick biofilm. We observed a two-fold reduction in the expression of biofilm-associated genes comprising
, and
in the presence of phytomolecules indicating that phytomolecules modulate quorum sensing pathway rather than killing the bacteria. These phytomolecules were non-toxic and non-hemolytic and had anti-adhesion and anti-invasion properties. In addition, quercetin and naringenin were found to be highly effective compared to known quorum-sensing inhibitors terrein and furanone C-30. Thus, this study provides evidence that phytomolecules: quercetin and naringenin modulate the quorum-sensing pathway rather than killing the bacteria and can be used as an anti-quorum-sensing molecule for therapy against the pathogen. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Infectious Agents and Disease, a section of the journal Frontiers in Microbiology Reviewed by: Md. Ashrafudoulla, Chung-Ang University, Republic of Korea; Ashwini Chauhan, Tripura University, India Edited by: Jeffrey H. Withey, Wayne State University, United States |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2023.1133569 |