Bacterial components induce cytokine and intercellular adhesion molecules-1 and activate transcription factors in dermal fibroblasts

• Published in: Research in microbiology Vol. 154; no. 5; pp. 337 - 344
• Main Authors: Perfetto, Brunella, Donnarumma, Giovanna, Criscuolo, Daniela, Paoletti, Iole, Grimaldi, Elena, Tufano, Maria Antonietta, Baroni, Adone
• Format: Journal Article
• Language: English
• Published: Paris Elsevier SAS 01.06.2003; Elsevier
• Subjects: Adult; Autocrine Communication; Bacterial surface components; Biological and medical sciences; Cell Adhesion - drug effects; Cytokine-adhesion molecules; Cytokines - biosynthesis; Cytokines - genetics; Dermal fibroblasts; Female; Fibroblasts - drug effects; Fibroblasts - metabolism; Fibroblasts - microbiology; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Genetics; Humans; I-kappa B Proteins - metabolism; Inflammation; Intercellular Adhesion Molecule-1 - biosynthesis; Intercellular Adhesion Molecule-1 - genetics; Interleukin-6 - biosynthesis; Interleukin-6 - genetics; Interleukin-8 - biosynthesis; Interleukin-8 - genetics; Lipopolysaccharides - pharmacology; Lymphocytes - cytology; Microbiology; Middle Aged; NF-kappa B - metabolism; NF-KappaB Inhibitor alpha; Paracrine Communication; Phosphorylation - drug effects; Porins - isolation & purification; Porins - pharmacology; Protein Processing, Post-Translational - drug effects; Pseudomonas aeruginosa - chemistry; Skin - cytology; Staphylococcal Protein A - pharmacology; Staphylococcus aureus - chemistry; Teichoic Acids - pharmacology; Transcription Factor AP-1 - metabolism; Transcription, Genetic; Tumor Necrosis Factor-alpha - biosynthesis; Tumor Necrosis Factor-alpha - genetics; Virology; Cytokine-adhesion molecules; Dermal fibroblasts; Bacterial surface components; Human; Porin; Intercellular adhesion molecule 1; Cytokine; Inflammatory disease; Infection; Gram positive bacteria; Bacteriosis; Lipopolysaccharide; Transcription factor; Fibroblast; Gram negative bacteria
• ISSN: 0923-2508; 1769-7123
• DOI: 10.1016/S0923-2508(03)00084-6

This study investigated the effect of various structural components of Gram-positive (lipotheichoic acid and protein A) and Gram-negative (porins and lipopolysaccharide) bacteria on human dermal fibroblasts. Fibroblasts are important effector cells which have a potential role in augmenting the inflammatory response in various diseases. In this study we present a profile of TNF-α, IL-6 and IL-8, the expression of intercellular adhesion molecules (ICAM-1) and the activation of transcriptional nuclear factor NF-kB and AP-1 in human dermal fibroblasts stimulated by bacterial surface components. Compared to the controls, increased ICAM–1, IL-6 and IL-8 gene expression after stimulation of LPS and porins at 2 and 4 h was more evident than that obtained following stimulation of LTA and PA. Gene expression was also associated with the production of cytokine proteins in culture supernatants. TNF-α gene expression remained undetectable. Moreover, LPS and porin treatments determined IkBα phosphorylation and degradation in human dermal fibroblasts and the subsequent activation of nuclear factors NF-kB and AP-1. These data suggest the importance of such stimuli in the first step of the inflammatory process, as well as the important role played by fibroblasts in skin inflammatory disease.