Lipolysis and thermogenesis in adipose tissues as new potential mechanisms for metabolic benefits of dietary fiber

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Vol. 33; pp. 118 - 124
• Main Authors: Han, Shu-Fen, Ph.D., M.D, Jiao, Jun, M.M, Zhang, Wei, M.M, Xu, Jia-Ying, Ph.D, Zhang, Weiguo, Ph.D., M.D, Fu, Chun-Ling, Ph.D, Qin, Li-Qiang, Ph.D., M.D
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2017; Elsevier Limited
• Subjects: Adipocytes; Adipocytes - drug effects; Adipocytes - metabolism; Adipose tissue; Adipose Tissue, Brown - drug effects; Adipose Tissue, Brown - metabolism; Adipose Tissue, Brown - physiology; Adipose Tissue, White - drug effects; Adipose Tissue, White - metabolism; Animals; Avena; Biotechnology industry; Body weight; Carbohydrates; Cardiovascular disease; Cyclic AMP - metabolism; Cyclic AMP-Dependent Protein Kinases - metabolism; Diabetes; Diet; Diet, High-Fat; Dietary Fats - adverse effects; Dietary Fats - metabolism; Dietary fiber; Dietary Fiber - pharmacology; Edible Grain - chemistry; Expenditures; Fibroblast Growth Factors; Gastroenterology and Hepatology; Immunoglobulins; Intervention; Intra-Abdominal Fat - drug effects; Intra-Abdominal Fat - metabolism; Kinases; Laboratory animals; Lipase - metabolism; Lipids; Lipolysis; Lipolysis - drug effects; Male; Metabolic disorders; Mice, Inbred C57BL; Obesity; Obesity - metabolism; Obesity - physiopathology; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism; Protein expression; Proteins; Receptors, Adrenergic, beta-3; Sterol Esterase - metabolism; Studies; Thermogenesis; Thermogenesis - drug effects; Transcription factors; Triticum; Triticum aestivum; Uncoupling Protein 1 - metabolism; Weight control; Wheat bran; China; Obesity; ： Thermogenesis; Dietary fiber; Lipolysis
• ISSN: 0899-9007; 1873-1244
• DOI: 10.1016/j.nut.2016.05.006

Abstract Objective Dietary fiber consumption is associated with reduced risk of the development of non-communicable diseases. The present study aimed to evaluate the effects of cereal dietary fiber on the levels of proteins involved in lipolysis and thermogenesis in white adipose tissue (WAT) and brown adipose tissue (BAT) of C57BL/6J mice fed a high-fat diet. Methods Male C57BL/6J mice were fed normal chow diet (Chow), high-fat diet (HFD), HFD plus oat fiber (H-oat) or HFD plus wheat bran fiber (H-wheat) for 24 wk. Body weight and food intake were recorded weekly. Serum adiponectin was assayed by an Elisa kit. Western blotting was used to assess the protein expressions of adipose triglyceride lipase (ATGL), cAMP protein kinase catalytic subunit (cAMP), proteinkinase A (PKA), perilipin A, hormone sensitive lipase (HSL), uncoupling protein 1 (UCP1), fibroblast growth factor 21 (FGF-21), β3-adrenergic receptor (β3-AR) and proliferator-activated receptor gamma coactivator-1α (PGC-1α) in the WAT and BAT. Results At the end of feeding period, body and adipose tissues weight in both H-oat and H-wheat groups were lower than that in the HFD group. Mice in the two groups of H-oat and H-wheat showed an increasing trend in serum adiponectin level. Compared with HFD group, cereal dietary fiber increased protein expressions involved in the lipolysis and browning process. Compared with H-wheat group, H-oat was more effective in protein expressions of PKA, PGC-1α and UCP1 of the WAT samples. Compared with H-oat group, H-wheat was more effective in protein expressions of PKA, ATGL, UCP1, β3-AR and FGF-21 of the BAT samples. Conclusions Taken together, our results suggest that cereal dietary fiber enhanced adipocyte lipolysis by cAMP-PKA-HSL pathway and promoted WAT browning by activation of UCP1, and consequently reduced visceral fat mass in response to HFD feeding.