Gut microbiome and metabolome to discover pathogenic bacteria and probiotics in ankylosing spondylitis

• Published in: Frontiers in immunology Vol. 15; p. 1369116
• Main Authors: Lai, Yupeng, Tang, Wenli, Luo, Xiao, Zheng, Huihui, Zhang, Yanpeng, Wang, Meiying, Yu, Guangchuang, Yang, Min
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 22.04.2024
• Subjects: Adult; ankylosing spondylitis; Bacteria - classification; Bacteria - isolation & purification; Bacteria - metabolism; biotin; CaCo-2; Feces - microbiology; Female; Gastrointestinal Microbiome; Humans; Immunology; Male; Metabolome; Metabolomics; Metagenomics - methods; microbiome; Middle Aged; Probiotics; Prospective Studies; Ruminococcus gnavus; Spondylitis, Ankylosing - immunology; Spondylitis, Ankylosing - metabolism; Spondylitis, Ankylosing - microbiology; Tumor Necrosis Factor Inhibitors - pharmacology; Tumor Necrosis Factor Inhibitors - therapeutic use; Bacteroides uniformis; CaCo-2; Ruminococcus gnavus; ankylosing spondylitis; biotin; oxypurinol; microbiome; metabolome
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2024.1369116

Previous research has partially revealed distinct gut microbiota in ankylosing spondylitis (AS). In this study, we performed non-targeted fecal metabolomics in AS in order to discover the microbiome-metabolome interface in AS. Based on prospective cohort studies, we further explored the impact of the tumor necrosis factor inhibitor (TNFi) on the gut microbiota and metabolites in AS. To further understand the gut microbiota and metabolites in AS, along with the influence of TNFi, we initiated a prospective cohort study. Fecal samples were collected from 29 patients with AS before and after TNFi therapy and 31 healthy controls. Metagenomic and metabolomic experiments were performed on the fecal samples; moreover, validation experiments were conducted based on the association between the microbiota and metabolites. A total of 7,703 species were annotated using the metagenomic sequencing system and by profiling the microbial community taxonomic composition, while 50,046 metabolites were identified using metabolite profiling. Differential microbials and metabolites were discovered between patients with AS and healthy controls. Moreover, TNFi was confirmed to partially restore the gut microbiota and the metabolites. Multi-omics analysis of the microbiota and metabolites was performed to determine the associations between the differential microbes and metabolites, identifying compounds such as oxypurinol and biotin, which were correlated with the inhibition of the pathogenic bacteria and the promotion of the probiotic bacteria . Through experimental studies, the relationship between microbes and metabolites was further confirmed, and the impact of these two types of microbes on the enterocytes and the inflammatory cytokine interleukin-18 (IL-18) was explored. In summary, multi-omics exploration elucidated the impact of TNFi on the gut microbiota and metabolites and proposed a novel therapeutic perspective: supplementation of compounds to inhibit potential pathogenic bacteria and to promote potential probiotics, therefore controlling inflammation in AS.