Biochemical investigations during in vitro adventitious shoot regeneration in leaflet explants from nodal segments of a mature Albizia procera tree

• Published in: Journal of forestry research Vol. 27; no. 3; pp. 699 - 705
• Main Authors: Rai, Ekta, Bouddha, Sulochna, Ansari, Shamim Akhtar
• Format: Journal Article
• Language: English
• Published: Harbin Northeast Forestry University 01.06.2016; Springer; Genetics and Plant Propagation Division, Tropical Forest Research Institute, Jabalpur 482021, India
• Subjects: 2,4-D; adults; adventitious shoots; Albizia procera; Biomedical and Life Sciences; callus; explants; Forestry; indole butyric acid; juveniles; Life Sciences; MS培养基; nitrate reductase; Original Paper; peroxidase; phenol; seedlings; sucrose; trees; 不定芽分化; 再生过程; 叶外植体; 成熟; 生化; 白格; 茎段; Adventitious shoot differentiation; Phenol; Nitrate reductase activity; Peroxidase activity; Soluble sugar
• ISSN: 1007-662X; 1993-0607
• DOI: 10.1007/s11676-015-0196-8

The in vitro adventitious shoot differentiation in leaflet explants of an adult tree differed from that of leaflet explants of seedlings of Albizia procera（Roxb.）Benth. reported previously elsewhere. The leaflet explants from an adult tree passed through an initial callus phase for30 days on MS medium supplemented with 3 % sucrose,2.5 l M 2,4-D followed by a subsequent adventitious shoot differentiation phase for another 30 days on half MS medium supplemented with 0.25 l M each of BA and IBA.The regeneration rate of in vitro adventitious shoots in explants from the adult tree, i.e.1.66 shoots/callus, was lower than that from seedlings, i.e. [10 shoots/callus,which was reported elsewhere. Correspondingly, the activities of nitrate reductase and peroxidase, and endogenous phenol content remained very low during in vitro adventitious shoot differentiation in leaflet explants of an adult tree possibly due to lower availability of competent stem（juvenile） cells for the process.