Assessment of the safety and probiotic characteristics of Lactobacillus salivarius CGMCC20700 based on whole-genome sequencing and phenotypic analysis
Lactic acid bacteria are generally regarded as alternatives to antibiotics in livestock and poultry farming, especially strains, which are safe and have probiotic potential. Although has long been proposed to be a probiotic, the understanding of the roles of this species is still in its infancy. Her...
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Published in | Frontiers in microbiology Vol. 14; p. 1120263 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
16.03.2023
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Subjects | |
Online Access | Get full text |
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Summary: | Lactic acid bacteria are generally regarded as alternatives to antibiotics in livestock and poultry farming, especially
strains, which are safe and have probiotic potential. Although
has long been proposed to be a probiotic, the understanding of the roles of this species is still in its infancy. Here, a strain of
CGMCC20700 isolated from the intestinal mucosa of Yunnan black-bone chicken broilers was investigated in the context of its safety and probiotic characteristics by whole-genome sequencing in parallel with phenotypic analysis. Whole-genome sequencing results showed that
CGMCC20700 has a single scaffold of 1,737,577 bp with an average guanine-to-cytosine (GC) ratio of 33.51% and 1,757 protein-coding genes. The annotation of Clusters of Orthologous Groups (COG) classified the predicted proteins from the assembled genome as possessing cellular, metabolic, and information-related functions. Sequences related to risk assessment, such as antibiotic resistance and virulence genes, were identified, and the strain was further confirmed as safe according to the results of antibiotic resistance, hemolytic, and acute oral toxicology tests. Two gene clusters of antibacterial compounds and broad-spectrum antimicrobial activity were identified using genome mining tools and antibacterial spectrum tests. Stress resistance genes, active stressor removal genes, and adhesion related genes that were identified and examined with various phenotypic assays (such as stress tolerance tests in acids and bile salts and auto aggregation and hydrophobicity assays). The strain showed a high survival rate in the presence of bile salts and under acidic conditions and exhibited significant auto aggregation capacity and hydrophobicity. Overall,
CGMCC20700 demonstrated excellent safety and probiotic potential at both the genomic and physiological levels and can be considered an appropriate candidate probiotic for livestock and poultry farming. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Naheed Mojgani, Razi Vaccine and Serum Research Institute, Iran; Jing Chen, Sichuan University, China; Hanghui Ye, University of Texas MD Anderson Cancer Center, United States Edited by: Renpeng Du, Heilongjiang University, China These authors have contributed equally to this work This article was submitted to Microorganisms in Vertebrate Digestive Systems, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2023.1120263 |