Preparation of a stationary phase with quaternary ammonium embedded group for selective separation of alkaloids based on ion-exclusion interaction

• Published in: Journal of separation science Vol. 35; no. 20; pp. 2685 - 2692
• Main Authors: Liu, Qiaoxia, Liang, Tu, Li, Kuiyong, Ke, Yanxiong, Jin, Yu, Liang, Xinmiao
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.10.2012; Wiley; Wiley Subscription Services, Inc
• Subjects: Alkaloids; Alkaloids - chemistry; Alkaloids - isolation & purification; Biological and medical sciences; Chains; Chromatography; Chromatography, Liquid - instrumentation; Chromatography, Liquid - methods; Cortex phellodendri; Cortexes; General pharmacology; Hydrophobic and Hydrophilic Interactions; Immobilization; Ion-exclusion; Medical sciences; Mixed-mode chromatography (MMC); Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Polymers; Quaternary Ammonium Compounds - chemistry; Quaternary ammonium embedded stationary phase; Resins, Synthetic - chemical synthesis; Resins, Synthetic - chemistry; Separation; Asia; China; Tandem mass spectrometry; Chemical analysis; Organic cation; Rutaceae; Bark; Mobile phase; Retention time; Ion exchange chromatography; Medicinal plant; Alkaloids; Alkaloid; Folk medicine; Dicotyledones; Phellodendron amurense; Angiospermae; pH; Mixed-mode chromatography (MMC); Ion exchange resin; Coupled method; Pharmacognosy; Phellodendron chinense; Quaternary ammonium compound; Ion-exclusion; Quaternary ammonium embedded stationary phase; Preparation; Plant origin; Spermatophyta; Mixed adsorbent; Bonded stationary phase; Cortex phellodendri
• ISSN: 1615-9306; 1615-9314; 1615-9314
• DOI: 10.1002/jssc.201200341

A stationary phase (named QA C10) with quaternary ammonium embedded between a propyl and a decyl chain was synthesized by immobilization of N,N‐dimethyldecylamine on chloropropyl–silica surface. A set of representative neutral, basic, and acidic compounds was employed to evaluate its chromatographic properties. The results illustrated that QA C10 was a mixed‐mode stationary phase possessing both hydrophobic and ionic characteristics. The QA C10 stationary phase was further used for selective separation of alkaloids from Cortex phellodendri. Under acidic condition, alkaloids could be eluted in first 8 min, while other neutral and acidic fractions were retained better on QA C10 column. Then, obtained alkaloid fraction was analyzed by LC‐MS/MS and 22 alkaloids were identified. Our study confirmed the advantages and application potential of the QA C10 stationary phase for alkaloids separation.