Calibration of a hand-held haemoglobin analyser for use on fish blood

The HemoCue haemoglobin analyser consistently overestimated haemoglobin concentration ([Hb]) in the blood of all fish species (sockeye salmon Oncorhynchus nerka, Chinook salmon Oncorhynchus tshawytscha, Pacific bluefin tuna Thunnus orientalis and chub mackerel Scomber japonicus) by 22–50% (9·9–36·0...

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Bibliographic Details
Published inJournal of fish biology Vol. 73; no. 10; pp. 2587 - 2595
Main Authors Clark, T. D., Eliason, E. J., Sandblom, E., Hinch, S. G., Farrell, A. P.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.12.2008
Subjects
Drabkin's method
haemoglobin concentration
haemoglobinometer
HemoCue Hb 201
Marine
Oncorhynchus nerka
Oncorhynchus tshawytscha
Scomber japonicus
spectrophotometer
Thunnus orientalis
Zoologi
Zoology
I, Pacific
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Summary:The HemoCue haemoglobin analyser consistently overestimated haemoglobin concentration ([Hb]) in the blood of all fish species (sockeye salmon Oncorhynchus nerka, Chinook salmon Oncorhynchus tshawytscha, Pacific bluefin tuna Thunnus orientalis and chub mackerel Scomber japonicus) by 22–50% (9·9–36·0 g l−1) over a [Hb] range of 20–160 g l−1. The systematic nature of the overestimation, however, allowed the formulation of an accurate calibration equation that can be used to correct values of [Hb] measured by the HemoCue in field studies.
Bibliography:istex:E4EB5A1A355A18DD6F6FDF6EC14A307D6AFB03DF
ArticleID:JFB2109
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content type line 23
ISSN:0022-1112
1095-8649
DOI:10.1111/j.1095-8649.2008.02109.x