Different adhesion types and active sensitivity of platelet subpopulations

• Published in: American journal of hematology Vol. 52; no. 4; pp. 322 - 324
• Main Authors: Zhao, B., Filler, T. J., Rickert, C. H., Liu, J. F., Skholl‐Engberts, A. D., Gurevich, V.
• Format: Journal Article
• Language: English
• Published: New York Wiley Subscription Services, Inc., A Wiley Company 01.08.1996; Wiley-Liss
• Subjects: Adenosine - pharmacology; adenosine binding; Biological and medical sciences; blood platelets; Blood Platelets - drug effects; Blood Platelets - physiology; Chromatography, High Pressure Liquid; Drug Resistance; Hematology; heterogeneity; Humans; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; platelet adhesion; Platelet Adhesiveness - drug effects; serotonin; Serotonin - secretion; Thrombin - pharmacology; Human; Heterogeneity; Adenosine; Purine nucleoside; Platelet; Serotonin; Phase contrast microscopy; Adhesion; Cell subpopulation
• ISSN: 0361-8609; 1096-8652
• DOI: 10.1002/(SICI)1096-8652(199608)52:4<322::AID-AJH15>3.0.CO;2-F

Two different adhesion patterns of blood platelets with different sensitivity to adenosine were demonstrated by means of reflection contrast microscopy with consecutive image analysis and cell affinity chromatography. High‐performance liquid chromatography revealed that thrombin‐induced serotonin release of adenosine‐sensitive platelets was lower than that of adenosine‐resistant cells. Our results indicate platelet heterogeneity and suggest that the platelets with lower adenosine sensitivity may be actively involved in the early interaction between platelets and injured endothelium. © 1996 Wiley‐Liss, Inc.