Optimization of microwave-assisted extraction of protopine and allocryptopine from stems of Macleaya cordata (Willd) R. Br. using response surface methodology

• Published in: Journal of separation science Vol. 33; no. 14; pp. 2160 - 2167
• Main Authors: Zhong, Ming, Huang, Ke-Long, Zeng, Jian-Guo, Li, Shuang, She, Jin-Ming, Li, Guiyin, Zhang, Li
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.07.2010; WILEY-VCH Verlag; WILEY‐VCH Verlag; Wiley
• Subjects: Allocryptopine; Analgesics, Opioid - chemistry; Analgesics, Opioid - isolation & purification; Benzophenanthridines - chemistry; Benzophenanthridines - isolation & purification; Berberine Alkaloids - chemistry; Berberine Alkaloids - isolation & purification; Biological and medical sciences; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Ethanol; Ethyl alcohol; Extraction; General pharmacology; Hydrogen-Ion Concentration; Macleaya cordata (Willd) R. Br; Mathematical models; Medical sciences; Medicine, Chinese Traditional; Microwave-assisted extraction; Microwaves; Molecular Structure; Optimization; Papaveraceae - chemistry; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Protopine; Response surface methodology; Solvents; Solvents - chemistry; Stems; Asia; China; Allocryptopine; Microwave-assisted extraction; Protopine; HPLC chromatography; Response surface methodology; Solvent extraction; Optimization; Papaveraceae; Stem; Medicinal plant; Alkaloid; Sample preparation; Folk medicine; Dicotyledones; Angiospermae; Response surface; Aromatic compound; Quantitative analysis; Berbine derivatives; Oxygen nitrogen heterocycle; Polyethylene; Pharmacognosy; Microwave assisted extraction; Microwave heating; Ketone; Operating conditions; Ultraviolet detector; Experimental design; Macleaya cordata (Willd) R. Br; Plant origin; Spermatophyta; Chemometrics
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201000085

The purpose of the research was to investigate the multiple response optimizations for the extraction of protopine and allocryptopine from the stems of Macleaya cordata (Willd) R. Br. by using microwave-assisted extraction (MAE). A three-level, three-factor Box-Behnken design of response surface methodology was used to develop response model, and desirability function was employed to optimize the effects of main extraction parameters. Three variables, ethanol concentration (20-80%, v/v), extraction temperature (30-70°C) and solvent/solid ratio (10:1 to 30:1, mL/g), were investigated in this study. The results showed that the optimum parameters of MAE were ethanol concentration of 45.2 % (v/v), extraction temperature of 54.7°C and solvent/solid ratio of 20.4:1 (mL/g). Under these conditions, the extraction yields of protopine and allocryptopine were 89.4 and 102.0%, respectively, and the extracta sicca yield was 12.5%. The combination use of response surface methodology, Box-Behnken design and the appropriate desirability function could provide an insight into a lab-scale MAE process, and help to develop procedures for commercial production of active ingredients from medical plants.