Proteomic analysis of the effects of baicalein on colorectal cancer cells

• Published in: Proteomics (Weinheim) Vol. 12; no. 6; pp. 810 - 819
• Main Authors: Huang, Wen-Shih, Kuo, Yi-Hung, Chin, Chih-Chien, Wang, Jeng-Yi, Yu, Hong-Ren, Sheen, Jiunn-Ming, Tung, Shui-Yi, Shen, Chien-Heng, Chen, Te-Chuan, Sung, Mei-Lan, Liang, Hwey-Fang, Kuo, Hsing-Chun
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.03.2012; Wiley-VCH; Wiley Subscription Services, Inc
• Subjects: Analytical, structural and metabolic biochemistry; Antioxidants - pharmacology; Apoptosis - drug effects; Baicalein; Biological and medical sciences; Cell biology; Cell cycle; Cell growth; Cell Line, Tumor; Cell Proliferation - drug effects; Colorectal cancer; Colorectal cancer cells; Colorectal Neoplasms - drug therapy; Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; Flavanones - pharmacology; Fundamental and applied biological sciences. Psychology; Gastroenterology. Liver. Pancreas. Abdomen; Gene Expression Regulation, Neoplastic - drug effects; Gene Knockdown Techniques; Humans; MALDI-TOF-TOF; Medical sciences; Miscellaneous; Peroxiredoxin VI - genetics; Peroxiredoxin VI - metabolism; Proteins; Proteome - analysis; Proteome - genetics; Proteomic differential displays; Proteomics; Reactive oxygen species; Reactive Oxygen Species - metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumors; Reactive oxygen species; Colorectal cancer cells; Rectal disease; Colorectal cancer; Cell biology; Malignant tumor; Matrix assisted laser desorption ionization; Colonic disease; Proteomics; MALDI-TOF-TOF; Baicalein; Proteomic differential displays; Digestive diseases; Intestinal disease; Cancer
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.201100270

Baicalein is the flavonoids with multiple pharmacological activities. The aim of our study was to investigate the effects of baicalein on colorectal cancer (CRC) and to recognize the targets of baicalein treatment. To better understand baicalein's target, proteomic approaches were used to purify and identify the protein substrates using 2D difference gel electrophoresis (2D SDS‐PAGE) to elucidate proteins differential display. Results from this study investigate that baicalein treatment of CRC cells results in reduced cell proliferation. As a result, differential protein displays between baicalein‐treated and untreated CRC were determined and validated. There were 11 differentially expressed proteins between baicalein‐treated and untreated CRC. Furthermore, we demonstrate that baicalein inhibits cancer cell proliferation and reduced reactive oxygen species (ROS) by up‐regulating the levels of peroxiredoxin‐6 (PRDX6). Knockdown of PRDX6 in baicalein‐treated CRC cells by specific small interfering RNA resulted in ROS production and proliferation, opposite of the baicalein treatment scenario as indicated by cell cycle distribution. These results illustrate that baicalein up‐regulates the expression of PRDX6, which attenuates the generation of ROS and inhibits the growth of CRC cells, whereas baicalein treatment have no effect on normal epithelial cells.