Electroporation in a Model of Cardiac Defibrillation

• Published in: Journal of cardiovascular electrophysiology Vol. 12; no. 12; pp. 1393 - 1403
• Main Authors: ASHIHARA, TAKASHI, YAO, TAKENORI, NAMBA, TSUNETOYO, ITO, MAKOTO, IKEDA, TAKANORI, KAWASE, AYAKA, TODA, SUNAO, SUZUKI, TORU, INAGAKI, MASASHI, SUGIMACHI, MASARU, KINOSHITA, MASAHIKO, NAKAZAWA, KAZUO
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Inc 01.12.2001
• Subjects: Action Potentials; Anisotropy; Arrhythmias, Cardiac - physiopathology; Arrhythmias, Cardiac - therapy; bidomain model; Computer Simulation; Electric Countershock; electrical shock; electroporation; Electroporation - instrumentation; Electroporation - methods; Humans; Membrane Potentials; Models, Cardiovascular; Myocardium - cytology; Myocardium - pathology; spiral wave; upper limit of vulnerability; ventricular fibrillation; virtual electrode
• ISSN: 1045-3873; 1540-8167
• DOI: 10.1046/j.1540-8167.2001.01393.x

Electroporation in a Model of Cardiac Defibrillation. Introduction: It is known that highstrength shock disrupts the lipid matrix of the myocardial cell membrane and forms reversible aqueous pores across the membrane. This process is known as “electroporation.” However, it remains unclear whether electroporation contributes to the mechanism of ventricular defibrillation. The aim of this computer simulation study was to examine the possible role of electroporation in the success of defibrillation shock. Methods and Results: Using a modified Luo‐Rudy‐1 model, we simulated two‐dimensional myocardial tissue with a homogeneous bidomain nature and unequal anisotropy ratios. Spiral waves were induced by the S1‐S2 method. Next, monophasic defibrillation shocks were delivered externally via two line electrodes. For nonelectroporating tissue, termination of ongoing fibrillation succeeded; however, new spiral waves were initiated, even with high‐strength shock (24 V/cm). For electroporating tissue, high‐strength shock (24 V/cm) was sufficient to extinguish ongoing fibrillation and did not initiate any new spiral waves. Weak shock (16 to 20 V/cm) also extinguished ongoing fibrillation; however, in contrast to the highstrength shock, new spiral waves were initiated. Success in defibrillation depended on the occurrence of electroporation‐mediated anodal‐break excitation from the physical anode and the virtual anode. Some excitation wavefronts following electrical shock used a deexcited area with recovered excitability as a pass‐through point; therefore, electroporation‐mediated anodal‐break excitation is necessary to block out the pass‐through point, resulting in successful defibrillation. Conclusion: The electroporation‐mediated anodal‐break excitation mechanism may play an important role in electrical defibrillation.