Interleukin-10 gene promoter polymorphisms and their protein production in pleural fluid in patients with tuberculosis

• Published in: FEMS immunology and medical microbiology Vol. 62; no. 1; pp. 84 - 90
• Main Authors: Liang, Li, Zhao, Yan-Lin, Yue, Jun, Liu, Jian-Fang, Han, Min, Wang, Hongxiu, Xiao, Heping
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.2011; Blackwell; Oxford University Press
• Subjects: Alleles; Biological and medical sciences; Cytokines; Enzyme-linked immunosorbent assay; Fundamental and applied biological sciences. Psychology; Gene Frequency; Gene polymorphism; Genotype; Humans; Interleukin 10; Interleukin-10 - genetics; Interleukin-10 - metabolism; Microbiology; Pleural Effusion - metabolism; Pleural fluid; pleural tuberculosis; Polymorphism; Polymorphism, Single Nucleotide; Promoter Regions, Genetic - genetics; protein production; Proteins; Tuberculosis; Tuberculosis, Pleural - diagnosis; Tuberculosis, Pleural - genetics; Tuberculosis, Pleural - immunology; Tuberculosis, Pleural - metabolism; gene polymorphism; pleural fluid; interleukin-10; pleural tuberculosis; protein production; Human; Respiratory disease; Pleural tuberculosis; Cytokine; Protein; Immunology; Gene; Interleukin 10; Polymorphism
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1111/j.1574-695X.2011.00791.x

Abstract Associations of interleukin-10 (IL-10) gene promoter polymorphisms and pleural tuberculosis risk remain unclear. The objective of this study was to determine IL-10 gene promoter polymorphisms at -1082, -819 and -592 sites and their protein production in pleural fluid (PF) in patients with and without pleural tuberculosis. IL-10 gene promoter polymorphisms at the -1082, -819 and -592 sites were genotyped using a SNaPshot assay. Protein levels of IL-10 in PF were measured using an enzyme-linked immunosorbent assay. There were no significant differences in the genotype and allele frequencies of IL-10 gene promoter polymorphisms at position -1082 between the pleural tuberculosis and the control groups. However, the frequency of -819 T or -592 A alleles was significantly more common in patients with pleural tuberculosis than controls. The protein levels of IL-10 in PF were statistically higher in the pleural tuberculosis group than in the control group. Moreover, the polymorphisms at the -1082, -819 and -592 sites were associated with protein levels of IL-10 in PF in the pleural tuberculosis group, while in the control group, only the polymorphism at position -1082 correlated with the protein levels. These findings support the association between IL-10 promoter polymorphisms at -819 and -592 sites and their protein production with pleural tuberculosis risk.