Biological Properties of Allogenic Articular Chondrocytes on the Surface of Bovine Cartilage Explants in vitro
Summary Bovine cartilage explants were co‐cultured with or without allogenic chondrocytes for 4 weeks. The attachment of the applied chondrocytes to cartilage after labelling with fluorescence was assessed using a confocal laser microscope. Morphological changes and the production of extracellular m...
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Published in | Journal of veterinary medicine. Series A Vol. 50; no. 8; pp. 418 - 423 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin, Germany
Blackwell Verlag GmbH
01.10.2003
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Subjects | |
Online Access | Get full text |
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Summary: | Summary
Bovine cartilage explants were co‐cultured with or without allogenic chondrocytes for 4 weeks. The attachment of the applied chondrocytes to cartilage after labelling with fluorescence was assessed using a confocal laser microscope. Morphological changes and the production of extracellular matrix (ECM) of co‐cultured chondrocytes on intact and damaged surfaces of cartilage were evaluated by histological and immunohistochemical methods. Co‐cultured chondrocytes attached to and proliferated on the intact and damaged areas of cartilage, and a new layer was created there. The defects were also filled with ECM produced by the co‐cultured chondrocytes. Glycosaminoglycans and collagen type II were detected in the newly formed ECM, and large numbers of rounded chondrocytes were observed at primitive lacunae in this matrix at 4 weeks of culture. The results suggest that chondrocytes have the ability to attach to, to proliferate on and to establish a new matrix on the intact and damaged surfaces of cartilage explants. |
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Bibliography: | ArticleID:TBED570 istex:4FD2A08CC575FC4F2E679BE741DA3F91EA659B25 ark:/67375/WNG-DKPK2LC1-L ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0931-184X 1439-0442 |
DOI: | 10.1046/j.1439-0442.2003.00570.x |