Identification and characterization of an indigoidine-like gene for a blue pigment biosynthesis in Streptomyces aureofaciens CCM 3239

• Published in: Folia microbiologica Vol. 55; no. 2; pp. 119 - 125
• Main Authors: Novakova, R., Odnogova, Z., Kutas, P., Feckova, L., Kormanec, J.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.03.2010; Springer Nature B.V
• Subjects: Applied Microbiology; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biomedical and Life Sciences; Biosynthesis; Environmental Engineering/Biotechnology; Erwinia chrysanthemi; Gene expression; Gene Expression Regulation, Bacterial; Genomics; Immunology; Life Sciences; Microbiology; Molecular Sequence Data; Pigments; Pigments, Biological - biosynthesis; Piperidones - metabolism; Streptomyces aureofaciens; Streptomyces aureofaciens - genetics; Streptomyces aureofaciens - metabolism; Streptomyces lavendulae; Polyketide; Streptomyces; Blue Pigment; Prodigiosin; Geosmin
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/s12223-010-0018-5

An incomplete oligoketide (PK; ‘polyketide’) gene cluster, aur1 , responsible for the production of an angucycline-like antibiotic auricin was identified in Streptomyces aureofaciens CCM 3239. A region downstream of the aur1 was cloned and sequenced, revealing 28 new genes encoding putative protein products involved in deoxysugar biosynthesis and other putative PK-related biosynthetic functions. In addition, a gene, bpsA , encoding a protein similar to non-ribosomal peptide synthetases (NRPSs) was identified in this region. A deduced protein product of the gene showed the highest similarity to NRPSs IndC from Erwinia chrysanthemi and BpsA from Streptomyces lavendulae , both involved in the biosynthesis of a blue pigment indigoidine. S. aureofaciens CCM 3239 was found to produce an extracellular blue pigment with identical properties as indigoidine. A deletion mutant of bpsA in S. aureofaciens CCM 3239 failed to produce the blue pigment. In addition, the deletion of bpsA had a positive effect on auricin production. The results indicate the involvement of the bpsA gene in biosynthesis of the indigoidine blue pigment in S. aureofaciens CCM 3239.