Establishment and proteomic characterization of patient-derived clear cell sarcoma xenografts and cell lines

• Published in: In vitro cellular & developmental biology. Animal Vol. 54; no. 2; pp. 163 - 176
• Main Authors: Sakumoto, Marimu, Oyama, Rieko, Takahashi, Mami, Takai, Yoko, Kito, Fusako, Shiozawa, Kumiko, Qiao, Zhiwei, Endo, Makoto, Yoshida, Akihiko, Kawai, Akira, Kondo, Tadashi
• Format: Journal Article
• Language: English
• Published: New York Springer Science & Business Media LLC 01.02.2018; Springer US; Society for In Vitro Biology
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Biopsy; Biotechnology; Cancer therapies; Cell Biology; Cell Culture; Colonies; Developmental Biology; Doxorubicin; Drug development; Drugs; ESTABLISHMENT OF CELL LINES; Fusion protein; Gene sequencing; Genes; Kinases; Life Sciences; Lymphatic system; Malignancy; Mass spectrometry; Mass spectroscopy; Medical prognosis; Melanoma; Mesenchyme; Metastasis; Patients; Polymerase chain reaction; Protein-tyrosine kinase; Sarcoma; Skin cancer; Stem Cells; Surgery; Therapeutic applications; Tissues; Tumor cell lines; Tumorigenesis; Tumors; Tyrosine; Viability; Xenografts; Xenotransplantation; Clear cell sarcoma; Drug response; Proteome; Primary culture cells; Xenografts
• ISSN: 1071-2690; 1543-706X
• DOI: 10.1007/s11626-017-0207-5

Clear cell sarcoma (CCS) is an aggressive mesenchymal malignancy characterized by the unique chimeric EWS-ATF1 fusion gene. Patient-derived cancer models are essential tools for the understanding of tumorigenesis and the development of anti-cancer drugs; however, only a limited number of CCS cell lines exist. The objective of this study was to establish patient-derived CCS models. We established patient-derived CCS models from a 43-yr-old female patient. We prepared the patient-derived xenografts (PDXs) from tumor tissues obtained through biopsy or surgery and isolated stable cell lines from PDXs and the original tumor tissue. The presence of gene fusions was examined by RT-PCR, and Sanger sequencing. The established cell lines were characterized by short tandem repeat, viability, colony and spheroid formation, and invasion analyses. Differences in gene enrichment between the primary tumor and cell lines were examined by mass spectrometry and KEGG pathway analysis. The cell lines were maintained for more than 80 passages, and had tumorigenic characteristics such as colony and spheroid formation and invasion. Mass spectrometric proteome analysis demonstrated that the cell lines were enriched for similar but distinct molecular pathways, compared to those in the xenografts and original tumor tissue. Next, tyrosine kinase inhibitors were screened for their suppressive effects on viability. We found that ponatinib, vandetanib, and doxorubicin suppressed the growth of cell lines, and had equivalent IC50 values. Further in-depth investigation and understanding of drug-sensitivity mechanisms will be important for the clinical applications of our cell lines.