In Vitro Antioxidant Profile of Phenolic Acid Derivatives

• Published in: Free radical research Vol. 36; no. 6; pp. 711 - 716
• Main Authors: Cos, Paul, Rajan, Padinchare, Vedernikova, Irina, Calomme, Mario, Pieters, Luc, Vlietinck, Arnold J., Augustyns, Koen, Haemers, Achiel, Berghe, Dirk Vanden
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 01.06.2002; Taylor & Francis
• Subjects: Antioxidant; Antioxidants - pharmacology; Biphenyl Compounds; Caffeic Acid; Caffeic Acids - chemistry; Caffeic Acids - pharmacology; Cells, Cultured; Dopamine - analogs & derivatives; Dopamine - chemistry; Dopamine - pharmacology; Dpph; Drug Evaluation, Preclinical - methods; Epr Spectroscopy; Fibroblasts - drug effects; Fibroblasts - metabolism; Free Radical Scavengers - pharmacology; Humans; Hydroxybenzoates - chemistry; Hydroxybenzoates - pharmacology; Inhibitory Concentration 50; Lipid Peroxidation; Lipid Peroxidation - drug effects; Microsomes - drug effects; Microsomes - metabolism; Picrates - metabolism; Polyphenols; Quercetin - analogs & derivatives; Quercetin - pharmacology
• ISSN: 1071-5762; 1029-2470
• DOI: 10.1080/10715760290029182

Several caffeic acid esters isolated from propolis exhibit interesting antioxidant properties, but their in vivo use is compromised by hydrolysis of the ester bond in the gastrointestinal tract. Therefore, a series of caffeic acid amides were synthesized and their in vitro antioxidant profile was determined. A series of hydroxybenzoic acids, hydroxycinnamic acids, and the synthesized caffeic acid amides were tested for both their 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and microsomal lipid peroxidation-inhibiting activity. Some of the highly active antioxidants were further tested by means of electron paramagnetic resonance for their hydroxyl radical scavenging activity. Since a promising antioxidant compound should show a lipid peroxidation-inhibiting activity at micromolar level and a low cytotoxicity, the cytotoxicity of the phenolic compounds was also studied. In all the assays used, the caffeic acid anilides and the caffeic acid dopamine amide showed an interesting antioxidant activity.