Active and Passive Smoking and Lifestyle Determinants of 8-Oxo-7,8-Dihydro-2′-Deoxyguanosine Levels in Human Leukocyte DNA

• Published in: Cancer epidemiology, biomarkers & prevention Vol. 14; no. 12; pp. 2975 - 2977
• Main Authors: LODOVICI, Maura, CALDINI, Silvia, LUCERI, Cristina, BAMBI, Franco, BODDI, Vieri, DOLARA, Piero
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 01.12.2005
• Subjects: Adolescent; Adult; Analysis of Variance; Biological and medical sciences; Coffee; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - metabolism; DNA Damage; Humans; Italy; Leukocytes - chemistry; Life Style; Male; Medical sciences; Middle Aged; Regression Analysis; Smoking - blood; Tea; Tobacco Smoke Pollution; Tobacco, tobacco smoking; Toxicology; Tumors; Italy; Human; Cancerology; Deoxyguanosine; Leukocyte; DNA; Passive smoking; Genetics; Oxidation; Public health; Life style
• ISSN: 1055-9965; 1538-7755
• DOI: 10.1158/1055-9965.EPI-04-0906

We investigated the effects of smoking and exposure to environmental tobacco smoke (ETS) on oxidative DNA damage by measuring 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) levels in DNA of leukocytes of healthy donors (30 smokers, 29 nonsmokers, and 28 ETS-exposed subjects). Nonsmokers had lower 8-oxodGuo levels compared with smokers (5.94 ± 0.87 × 10 −6 and 19.85 ± 4.75 × 10 −6 2-deoxyguanosine, respectively, means ± SE, P = 0.00007). Subjects exposed to ETS had higher mean value of 8-oxodGuo compared with nonsmokers (9.18 ± 1.53 × 10 −6 2-deoxyguanosine, mean ± SE), nonsignificant by univariate analysis ( P = 0.074). Multiregression analysis indicated that the increase of 8-oxodGuo levels induced by ETS was significant ( P = 0.045) and that coffee and tea consumption reduced DNA oxidation ( P = 0.0053). Oxidative leukocyte DNA damage was positively correlated with plasma cotinine levels in ETS-exposed subjects ( r = 0.47, P < 0.01, n = 28) and was increased by age in nonsmokers and ETS-exposed subjects ( P = 0.049). The results seem to confirm that ETS exposure is capable of inducing some oxidative DNA damage in circulating leukocytes and that coffee and tea consumption might partially protect against smoking-induced oxidation damage. (Cancer Epidemiol Biomarkers Prev 2005;14(12):2975–7)