Current status of extended spectrum β-lactamase-producing Escherichia coli , Klebsiella pneumoniae and Proteus mirabilis in Okinawa prefecture, Japan

• Published in: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy Vol. 22; no. 5; pp. 281 - 286
• Main Authors: Nakama, Rika, Shingaki, Aoi, Miyazato, Hiroko, Higa, Rikako, Nagamoto, Chota, Hamamoto, Kouta, Ueda, Shuhei, Hachiman, Teruyuki, Touma, Yuki, Miyagi, Kazufumi, Kawahara, Ryuji, Toyosato, Takehiko, Hirai, Itaru
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.05.2016
• Subjects: Anti-Bacterial Agents - pharmacology; beta-Lactamases; CTX-M-type extended spectrum β-lactamase; Drug Resistance, Bacterial; Enterobacteriaceae; Enterobacteriaceae - drug effects; Enterobacteriaceae - enzymology; Enterobacteriaceae - pathogenicity; Enterobacteriaceae Infections - epidemiology; Enterobacteriaceae Infections - microbiology; Escherichia coli; Hematology, Oncology and Palliative Medicine; Humans; Japan - epidemiology; Klebsiella pneumoniae; Klebsiella pneumoniae possessing blaSHV; Microbial Sensitivity Tests; Prevalence; Proteus mirabilis; Klebsiella pneumoniae possessing bla SHV; Prevalence; CTX-M-type extended spectrum β-lactamase; Klebsiella pneumoniae possessing blaSHV; Klebsiella pneumoniae possessing bla(SHV)
• ISSN: 1341-321X; 1437-7780
• DOI: 10.1016/j.jiac.2016.01.008

Abstract Enterobacteriaceae producing extended spectrum β-lactamase (ESBL) are distributed worldwide. In this study, 114 ESBL-producing Enterobacteriaceae were isolated by analyzing 1672 clinical isolates of Enterobacteriaceae collected from an Okinawa prefectural hospital in Japan between June 2013 and July 2014. The overall prevalence of ESBL-producing Enterobacteriaceae was 6.8%; the prevalence of different bacterial species among the ESBL-producing isolates was as follows: 11.5% Escherichia coli (90 of 783 isolates), 6.2% Klebsiella pneumoniae (19 of 307 isolates), and 11.1% Proteus mirabilis (5 of 45 isolates). The ESBL types blaCTX-M-1 ,-3 ,-15 ,-2 ,-14 ,-27 , and mutants of blaSHV-1 were detected. Among them, blaCTX-M-15 (33.3%), blaCTX-M-14 (27.8%) and blaCTX-M-27 (33.3%) were dominant in the E. coli isolates, whereas a blaSHV mutant which possessed four mutations (Tyr7Phe, Leu35Gln, Gly238Ser and Glu240Lys) in the amino acid sequence of SHV-1 dominated in the K. pneumoniae isolates (11 of 19, 57.9%). The pandemic E. coli ST131 clone was found to constitute 3.3% of the overall examined isolates and 62.2% of the ESBL-producing E. coli isolates. Our results suggest that the genetic combination of blaCTX-M , and blaSHV and antibiotics-resistant profile were different from that in other regions such as other areas of Japan, Asia, Europe, and North America, especially in the ESBL-producing K. pneumoniae isolates and in the E. coli B2-O25b-ST131 isolates possessing blaCTX-M-15 (40.7% of the E. coli B2-O25b-ST131 isolates). Taken together, our results indicate that the ESBL-producing Enterobacteriaceae in Okinawa, Japan, might be of a unique nature.