Higher cytoplasmic and nuclear poly(ADP-ribose) polymerase expression in familial than in sporadic breast cancer

• Published in: Virchows Archiv : an international journal of pathology Vol. 461; no. 4; pp. 425 - 431
• Main Authors: Klauke, Marie-Luise, Hoogerbrugge, Nicoline, Budczies, Jan, Bult, Peter, Prinzler, Judith, Radke, Cornelia, van Krieken, J. Han J. M., Dietel, Manfred, Denkert, Carsten, Müller, Berit Maria
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.10.2012; Springer; Springer Nature B.V
• Subjects: Adenosine diphosphate; Adult; Aged; Antineoplastic Agents - therapeutic use; Biological and medical sciences; Biomarkers, Tumor - metabolism; Breast cancer; Breast Neoplasms - drug therapy; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Cell Nucleus - metabolism; Cell Nucleus - pathology; Cytoplasm - metabolism; Cytoplasm - pathology; Enzyme Inhibitors - therapeutic use; Female; Gene Expression Regulation, Neoplastic; Genetic Predisposition to Disease; Gynecology. Andrology. Obstetrics; Humans; Investigative techniques, diagnostic techniques (general aspects); Mammary gland diseases; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Neoplasm Grading; Original Article; Pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Poly(ADP-ribose) Polymerases - metabolism; Treatment Outcome; Tumors; Poly(ADP-ribose) polymerase; PARP; Breast cancer; Hereditary breast cancer; BRCA mutation; Breast disease; Sporadic; Enzyme; Transferases; Glycosyltransferases; Malignant tumor; Hereditary; NAD; Mammary gland diseases; Anatomic pathology; ADP-ribosyltransferase; Familial cancer; Genetics; Mutation; Pentosyltransferases; Cytoplasm; Cancer
• ISSN: 0945-6317; 1432-2307
• DOI: 10.1007/s00428-012-1311-2

Poly(ADP-ribose) polymerase 1 (PARP) is a key element of the single-base excision pathway for repair of DNA single-strand breaks. To compare the cytoplasmic and nuclear poly(ADP-ribose) expression between familial (BRCA1, BRCA2, or non BRCA1/2) and sporadic breast cancer, we investigated 39 sporadic and 39 familial breast cancer cases. The two groups were matched for hormone receptor status and human epidermal growth factor receptor 2 status. Additionally, they were matched by grading with a maximum difference of ±1 degree (e.g., G2 instead of G3). Cytoplasmic PARP (cPARP) expression was significantly higher in familial compared to sporadic breast cancer ( P  = 0.008, chi-squared test for trends) and a high nuclear PARP expression (nPARP) was significantly more frequently observed in familial breast cancer (64 %) compared with sporadic breast cancer (36 %) ( P  = 0.005, chi-squared test). The overall PARP expression was significantly higher in familial breast cancer ( P  = 0.042, chi-squared test). In familial breast cancer, a combination of high cPARP and high nPARP expression is the most common (33 %), whereas in sporadic breast cancer, a combination of low cPARP and intermediate nPARP expression is the most common (39 %). Our results show that the overall PARP expression in familial breast cancer is higher than in sporadic breast cancer which might suggest they might respond better to treatment with PARP inhibitors.