CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products

Lactic acid bacteria (LAB) plays a crucial role in aquatic products biopreservation as it can inhibit many bacteria, in particular the specific spoilage organisms (SSOs) of aquatic products, by competing for nutrients or producing one or more metabolites which have antimicrobial activity, such as ba...

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Bibliographic Details
Published inFrontiers in bioengineering and biotechnology Vol. 10; p. 1114588
Main Authors Dong, Huina, Wang, Huiying, Fu, Shaoping, Zhang, Dawei
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 23.12.2022
Subjects
aquatic products biopreservation
Bioengineering and Biotechnology
CRISPR/Cas system
genome editing
lactic acid bacteria
transcriptional regulation
CRISPR/Cas system
genome editing
lactic acid bacteria
transcriptional regulation
aquatic products biopreservation
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Summary:Lactic acid bacteria (LAB) plays a crucial role in aquatic products biopreservation as it can inhibit many bacteria, in particular the specific spoilage organisms (SSOs) of aquatic products, by competing for nutrients or producing one or more metabolites which have antimicrobial activity, such as bacteriocins. spp. and spp. are the most commonly used Lactic acid bacterias in aquatic products preservation. The improvement of gene editing tools is particularly important for developing new lactic acid bacteria strains with superior properties for aquatic products biopreservation. This review summarized the research progress of the most widely used CRISPR/Cas-based genome editing tools in spp. and spp. The genome editing tools based on homologous recombination and base editor were described. Then, the research status of CRISPRi in transcriptional regulation was reviewed briefly. This review may provide a reference for the application of CRISPR/Cas-based genome editing tools to other lactic acid bacteria species.
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Edited by: Bo Yu, Institute of Microbiology (CAS), China
These authors have contributed equally to this work
This article was submitted to Industrial Biotechnology, a section of the journal Frontiers in Bioengineering and Biotechnology
Zhen Kang, Jiangnan University, China
Reviewed by: Feiran Li, Chalmers University of Technology, Sweden
ISSN:2296-4185
2296-4185
DOI:10.3389/fbioe.2022.1114588