Genetic mutations of p53 and k-ras in gastric carcinoma patients from Hunan, China

• Published in: Tumor biology Vol. 32; no. 2; pp. 367 - 373
• Main Authors: Chen, Han-chun, Chen, Hui-juan, Khan, Md. Asaduzzaman, Rao, Zhou-zhou, Wan, Xin-xing, Tan, Bo, Zhang, Dian-zheng
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.04.2011; Springer Nature B.V
• Subjects: Adult; Aged; Alleles; Asian people; Biomedical and Life Sciences; Biomedicine; Cancer; Cancer Research; Case-Control Studies; China; Codon - genetics; Exons - genetics; Female; Frameshift Mutation - genetics; Gastrointestinal diseases; Genes, p53 - genetics; Genes, ras - genetics; Humans; Male; Middle Aged; Mutation; Point Mutation - genetics; Polymorphism; Population genetics; Research Article; Stomach Neoplasms - ethnology; Stomach Neoplasms - genetics; China; Gastric carcinoma; mutation; Single strand conformation polymorphism

This case–control study investigated the mutations in p53 and k-ras genes of 123 gastric carcinoma patients and 129 normal individuals from Hunan, China. By isolating genomic DNA from peripheral blood and employing polymerase chain reaction–single strand conformation polymorphism and DNA sequencing,...