Genetic mutations of p53 and k-ras in gastric carcinoma patients from Hunan, China

• Published in: Tumor biology Vol. 32; no. 2; pp. 367 - 373
• Main Authors: Chen, Han-chun, Chen, Hui-juan, Khan, Md. Asaduzzaman, Rao, Zhou-zhou, Wan, Xin-xing, Tan, Bo, Zhang, Dian-zheng
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.04.2011; Springer Nature B.V
• Subjects: Adult; Aged; Alleles; Asian people; Biomedical and Life Sciences; Biomedicine; Cancer; Cancer Research; Case-Control Studies; China; Codon - genetics; Exons - genetics; Female; Frameshift Mutation - genetics; Gastrointestinal diseases; Genes, p53 - genetics; Genes, ras - genetics; Humans; Male; Middle Aged; Mutation; Point Mutation - genetics; Polymorphism; Population genetics; Research Article; Stomach Neoplasms - ethnology; Stomach Neoplasms - genetics; China; Gastric carcinoma; mutation; Single strand conformation polymorphism
• ISSN: 1010-4283; 1423-0380
• DOI: 10.1007/s13277-010-0129-2

This case–control study investigated the mutations in p53 and k-ras genes of 123 gastric carcinoma patients and 129 normal individuals from Hunan, China. By isolating genomic DNA from peripheral blood and employing polymerase chain reaction–single strand conformation polymorphism and DNA sequencing, the mutations of p53 exons-5, 6, 7, and 8 and k-ras were detected. The overall mutation frequency of p53 was 29.3%, and mutation was found in all four exons studied. The point mutations were predominant and among them, G:C→A:T was the highest (41.7%), followed by A:T→G:C (25%), G:C→C:G (11.1%), G:C→T:A (8.3%), and A:T→T:A (2.8%). The frameshift mutation was 11.1%. Mutations were detected in codons-131, 132, 133, 135, 149, 151, 162, 167, 173, 174, and 175 of exon 5, codons-193, 197, 213, and 215 of exon 6, codons-245, 246, 248, 249, and 270 of exon 7, and codons-271, 272, 273, and 282 of exon 8 of p53 . The overall frequency of mutation in k-ras was 9.8%, mostly in codon-12 (91.7%) and in codon-13 (8.3%). There was no significant relationship between p53 and k-ras gene mutation in gastric carcinoma patients. Also, the relationships between p53 mutation and age, sex, smoking or drinking, and tumor metastasis were not significant. However, the patients with high/high-middle differentiated gastric carcinoma had a higher association with of p53 mutations. This study identified some novel p53 mutations in gastric cancer and showed mutation pattern and frequency of p53 and k-ras in the population of the central southern region of China.