Operating conditions optimization for (+)-terrein production in a stirred bioreactor by Aspergillus terreus strain PF-26 from marine sponge Phakellia fusca

• Published in: Bioprocess and biosystems engineering Vol. 35; no. 9; pp. 1651 - 1655
• Main Authors: Xu, Bailiang, Yin, Ying, Zhang, Fengli, Li, Zhiyong, Wang, Lianyun
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.11.2012; Springer; Springer Nature B.V
• Subjects: Aeration; Animals; Aspergillus - isolation & purification; Aspergillus - physiology; Biological and medical sciences; Biomass; Bioreactors; Biotechnology; Chemistry; Chemistry and Materials Science; Cyclopentanes - metabolism; Environmental Engineering/Biotechnology; Fluidized bed reactors; Food Science; Fundamental and applied biological sciences. Psychology; Fungi; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Metabolites; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Natural products; Optimization techniques; Porifera - microbiology; Short Communication; Spores, Fungal - growth & development; Various methods and equipments; Fermentation; Terrein; Stirred bioreactor; Optimization; Stirred tank reactor; Operating conditions; Fungi; Marine environment; Bioreactor; Porifera; Production; Fungi Imperfecti; Invertebrata; Aspergillus terreus
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-012-0735-z

Terrein is a fungal metabolite with application values in the fields of medicine, cosmetology, and agriculture. However, mass production of single configuration terrein is still a big challenge. In this study, operating factors such as inoculation, agitation speed, aeration rate, pH control, and nutrient feeding were preliminarily optimized to improve the (+)-terrein production in the 5-L stirred bioreactor from the marine sponge-derived fungus Aspergillus terreus PF-26. Spore inoculation, low agitation speed, and aeration rate were proved to be suitable for A. terreus PF-26 to produce (+)-terrein in the stirred bioreactor. At 50 rpm agitation speed and 0.33 vvm aeration rate, 2.68 g/L (+)-terrein was achieved by feeding twofold concentrated maltose and glucose medium on the sixth day and controlling pH at 4.5 from the fourth day. This study lays foundation for the mass production of (+)-terrein by the marine filamentous A. terreus s train PF-26 in the stirred bioreactor.