Downstream effects of striatal-enriched protein tyrosine phosphatase reduction on RNA expression in vivo and in vitro

• Published in: Neuroscience Vol. 278; pp. 62 - 69
• Main Authors: Reinhart, V.L, Nguyen, T, Gerwien, R, Kuhn, M, Yates, P.D, Lanz, T.A
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 10.10.2014
• Subjects: Animals; Corpus Striatum - metabolism; Gene Expression; HEK293 Cells; Hippocampus - metabolism; Humans; Lentivirus; Male; MAP Kinase Signaling System; Mice; Mice, Knockout; microarray; Nerve Growth Factors - genetics; Neurology; Neurons - metabolism; NMDA; Protein Tyrosine Phosphatases - genetics; PTPN5; qRT-PCR; Rats; RNA - metabolism; schizophrenia; Signal Transduction; STEP; extracellular signal-regulated kinase; dual specificity phosphatase 4 gene; N-methyl- d-aspartic acid; septin 11 gene; schizophrenia; Stxbp1; gene ontology; Ipw; shRNA; postsynaptic density 95; syntaxin-binding protein gene; Homozygous; gene of interest; HOM; qRT-PCR; Fos; GluR2; NR2B; Arc; associated protein gene; N-methyl- d-aspartate receptor subtype 2B; glutamate receptor, ionotropic, AMPA 2; Sik1; Tmem114; ERK; heterozygous; insulin-like growth factor 1 gene; long-term potentiation; RT; room temperature; Dulbecco’s modified Eagle’s medium; calcium/calmodulin-dependent protein kinase II alpha gene; LTP; knock-out; short hairpin ribonucleic acid; DMEM; Dusp4; analysis of variance; ANOVA; Camk2a; Pitpnm family member 3; NMDA; salt-inducible kinase 1 gene; WT; quantitative real-time polymerase chain reaction; GOI; transmembrane protein 114; Egr2; KO; GO; Pitpnm3; STEP; striatal-enriched protein tyrosine phosphatase; Igf1; Sept11; Early growth response 2; microarray; PSD95; FBJ murine osteosarcoma viral oncology homolog gene; HET; imprinted in Prader–Willi syndrome gene; wild-type; PTPN5
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2014.08.002

Highlights • Microarray data from STEP KO mice-identified pathways related to ERK signaling, neurodevelopment, and synaptic plasticity. • STEP KO induced expression of immediate-early genes which were validated in the striatum, cortex and hippocampus by RT-PCR. • Primary cortical neurons treated with STEP shRNA showed elevated DUSP4, FOS, SPP1, and STXBP1 mRNA. • Primary cortical neurons treated with STEP shRNA showed reduced CAMK2A, SEPT11 and TMEM114 mRNA.