Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

• Published in: Frontiers in bioengineering and biotechnology Vol. 11; p. 1020430
• Main Authors: Wang, Jingwen, Jiang, Han, Pan, Leiming, Gu, Xiuying, Xiao, Chaogeng, Liu, Pengpeng, Tang, Yulong, Fang, Jiehong, Li, Xiaoqian, Lu, Chenze
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 01.02.2023
• Subjects: Bioengineering and Biotechnology; biosensor; microfluidic chip; nucleic acid amplification; nucleic acid testing; rapid on-site detection; nucleic acid testing; biosensor; microfluidic chip; nucleic acid amplification; rapid on-site detection
• ISSN: 2296-4185; 2296-4185
• DOI: 10.3389/fbioe.2023.1020430

As nucleic acid testing is playing a vital role in increasingly many research fields, the need for rapid on-site testing methods is also increasing. The test procedure often consists of three steps: Sample preparation, amplification, and detection. This review covers recent advances in on-chip methods for each of these three steps and explains the principles underlying related methods. The sample preparation process is further divided into cell lysis and nucleic acid purification, and methods for the integration of these two steps on a single chip are discussed. Under amplification, on-chip studies based on PCR and isothermal amplification are covered. Three isothermal amplification methods reported to have good resistance to PCR inhibitors are selected for discussion due to their potential for use in direct amplification. Chip designs and novel strategies employed to achieve rapid extraction/amplification with satisfactory efficiency are discussed. Four detection methods providing rapid responses (fluorescent, optical, and electrochemical detection methods, plus lateral flow assay) are evaluated for their potential in rapid on-site detection. In the final section, we discuss strategies to improve the speed of the entire procedure and to integrate all three steps onto a single chip; we also comment on recent advances, and on obstacles to reducing the cost of chip manufacture and achieving mass production. We conclude that future trends will focus on effective nucleic acid extraction combined methods and direct amplification isothermal methods.