Duck TRIM29 negatively regulates type I IFN production by targeting MAVS

• Published in: Frontiers in immunology Vol. 13; p. 1016214
• Main Authors: Li, Weiqiang, Song, Yating, Du, Yuqing, Huang, Zhanhong, Zhang, Meng, Chen, Zuxian, He, Zhuoliang, Ding, Yangbao, Zhang, Junsheng, Zhao, Luxiang, Sun, Hailiang, Jiao, Peirong
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 06.01.2023
• Subjects: Animals; duck; Ducks; Gene Expression; Immunity, Innate; Immunology; innate immune response; Interferon Type I; Interferon-beta - metabolism; Mammals - metabolism; MAVS; TRIM29; Type I IFN; MAVS; TRIM29; innate immune response; duck; Type I IFN
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2022.1016214

The innate immune response is a host defense mechanism that induces type I interferon and proinflammatory cytokines. Tripartite motif (TRIM) family proteins have recently emerged as pivotal regulators of type I interferon production in mammals. Here, we first identified duck TRIM29, which encodes 571 amino acids and shows high sequence homology with other bird TRIM29 proteins. DuTRIM29 inhibited IFN-β and IRF7 promoter activation in a dose-dependent manner and downregulated the mRNA expression of IFN-β, IRF7, Mx and IL-6 mediated by duRIG-I. Moreover, duTRIM29 interacted and colocalized with duMAVS in the cytoplasm. DuTRIM29 interacted with duMAVS its C-terminal domains. In addition, duTRIM29 inhibited IFN-β and IRF7 promoter activation and significantly downregulated IFN-β and immune-related gene expression mediated by duMAVS in ducks. Furthermore, duTRIM29 induced K29-linked polyubiquitination and degradation of duMAVS to suppress the expression of IFN-β. Overall, our results demonstrate that duTRIM29 negatively regulates type I IFN production by targeting duMAVS in ducks. This study will contribute to a better understanding of the molecular mechanism regulating the innate immune response by TRIM proteins in ducks.