Heterologous surface display on lactic acid bacteria: non-GMO alternative?

• Published in: Bioengineered Vol. 6; no. 3; pp. 179 - 183
• Main Authors: Zadravec, Petra, Štrukelj, Borut, Berlec, Aleš
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 2015
• Subjects: Addenda; bacteria-like particles; Cell Surface Display Techniques - methods; Cell Wall - chemistry; cell-wall binding domains; EFSA; EMA; genetically-modified organism; gram-positive enhancer matrix; Lactobacillus - chemistry; Lactobacillus - classification; LysM; Organisms, Genetically Modified; Recombinant Fusion Proteins - chemistry; regulatory agency; surface display; cell-wall binding domains; LysM; bacteria-like particles; genetically-modified organism; gram-positive enhancer matrix; EMA; regulatory agency; surface display; EFSA
• ISSN: 2165-5979; 2165-5987
• DOI: 10.1080/21655979.2015.1040956

Lactic acid bacteria (LAB) are food-grade hosts for surface display with potential applications in food and therapy. Alternative approaches to surface display on LAB would avoid the use of recombinant DNA technology and genetically-modified organism (GMO)-related regulatory requirements. Non-covalent surface display of proteins can be achieved by fusing them to various cell-wall binding domains, of which the Lysine motif domain (LysM) is particularly well studied. Fusion proteins have been isolated from recombinant bacteria or from their growth medium and displayed on unmodified bacteria, enabling heterologous surface display. This was demonstrated on non-viable cells devoid of protein content, termed bacteria-like particles, and on various species of genus Lactobacillus. Of the latter, Lactobacillus salivarius ATCC 11741 was recently shown to be particularly amenable for LysM-mediated display. Possible regulatory implications of heterologous surface display are discussed, particularly those relevant for the European Union.