A monoclonal antibody-based dipstick assay for diagnosis of urinary schistosomiasis

• Published in: Transactions of the Royal Society of Tropical Medicine and Hygiene Vol. 91; no. 5; pp. 554 - 556
• Main Authors: Bosompem, Kwabena M., Ayi, Irene, Anyan, William K., Arishima, T., Nkrumah, Francis K., Kojima, Somei
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01.09.1997; Royal Society of Tropical Medicine and Hygiene; Elsevier
• Subjects: Adolescent; Adult; Antibodies, Monoclonal; Antigens, Helminth - urine; Biological and medical sciences; Child; Child, Preschool; diagnosis; dipstick test; Diseases caused by trematodes; Enzyme-Linked Immunosorbent Assay; Helminthic diseases; Humans; Infectious diseases; Medical sciences; Middle Aged; Parasitic diseases; Reagent Kits, Diagnostic; Schistosoma haematobium; Schistosomiases; schistosomiasis; Schistosomiasis haematobia - diagnosis; Schistosomiasis haematobia - immunology; Sensitivity and Specificity; Serologic Tests; Tropical medicine; Ghana; Africa; dipstick test; diagnosis; Schistosoma haematobium; schistosomiasis; enzyme-linked immunosorbent assay; Human; Urine; Urinary system disease; Trematode disease; Plathelmintha; Parasitosis; Helminthiasis; Trematoda; Infection; Antigen; Schistosomiasis; Helmintha; Diagnosis; Feces; Invertebrata; ELISA assay
• ISSN: 0035-9203; 1878-3503
• DOI: 10.1016/S0035-9203(97)90024-9

A Schistosoma haematobium species-specific mouse immunoglobulin (Ig) G1 monoclonal antibody (mab) Sh2/15.F that bound a 29 kDa peptide was utilized to develop a membrane-based dipstick enzyme-linked immunosorbent assay for specific diagnosis of urinary schistosomiasis. Strips of polyvinylidene difluoride membrane were wetted with methanol and stored in distilled water. The strips were used to capture urinary antigens which were then revealed by incubation in a mixture of specific mab and peroxidase-conjugated goat anti-mouse IgG. The assay correctly identified 26 30 (87%) of egg-negative control individuals and 53 54 (98%) of parasitologically confirmed cases including all of 6 individuals treated with praziquantel (40 mg/kg) but not cured. Also, the assay detected S. haematobium antigens in the urine of 3 individuals from whom 2 specimens had to be examined microscopically to confirm infection, thus suggesting that the mab detection method may have greater sensitivity than microscopy.