A novel regulatory element responsible for high transcriptional expression of acerola GDP-d-mannose pyrophosphorylase gene

• Published in: Bioscience, biotechnology, and biochemistry Vol. 81; no. 6; pp. 1194 - 1197
• Main Authors: Kondo, Takayuki, Fujikawa, Yukichi, Esaka, Muneharu
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03.06.2017; Oxford University Press
• Subjects: acerola; Arabidopsis - enzymology; Arabidopsis - genetics; ascorbic acid; Base Sequence; cis-element; Enhancer Elements, Genetic; GDP-d-mannose pyrophosphorylase; Gene Expression Regulation, Plant; Genes, Reporter; Luciferases - genetics; Luciferases - metabolism; Malpighiaceae - enzymology; Malpighiaceae - genetics; Nucleotidyltransferases - genetics; Nucleotidyltransferases - metabolism; Plant Leaves - enzymology; Plant Leaves - genetics; Plant Proteins - genetics; Plant Proteins - metabolism; Promoter Regions, Genetic; Protoplasts - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Transcription, Genetic; ascorbic acid; GDP; cis-element; mannose pyrophosphorylase; acerola; GDP-d-mannose pyrophosphorylase
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2017.1285690

GDP-d-mannose pyrophosphorylase (GMP) is one of the enzymes that highly expressed in acerola plants. A promoter assay suggests the presence of a new cis-element in the −1087 to −1083 bp sequence of the MgGMP promoter. Moreover, cis-elements, present in the −1080 to −600 bp sequence of the MgGMP promoter, function as enhancers of MgGMP expression.