HSP25 Affects the Proliferation and Differentiation of Rat Dental Follicle Cells

• Published in: International journal of oral science Vol. 1; no. 2; pp. 72 - 80
• Main Authors: Du, Yu, Gu, Hai‐jing, Gong, Qi‐mei, Yang, Fang, Ling, Jun‐qi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.06.2009; Springer Nature B.V; Nature Publishing Group
• Subjects: Alkaline Phosphatase - analysis; Ameloblasts - cytology; Animals; Cell Culture Techniques; Cell Differentiation - physiology; Cell Proliferation; Coloring Agents; Cytoplasm - ultrastructure; Dental Sac - cytology; Dental Sac - growth & development; Dentistry; Flow Cytometry; Fluorescent Antibody Technique, Indirect; HSP27 Heat-Shock Proteins - analysis; HSP27 Heat-Shock Proteins - physiology; Medicine; Odontoblasts - cytology; Oral and Maxillofacial Surgery; Original Scientific; original-scientific-article; Orthopedics; Rats; Rats, Sprague-Dawley; Surgical Orthopedics; Tetrazolium Salts; Thiazoles; Tooth Germ - cytology; Up-Regulation - physiology; 免疫荧光法; 口腔卫生; 碱性磷酸酶; 细胞质; cell differentiation; alkaline phosphatase (ALP); cell proliferation; HSP25; dental follicle
• ISSN: 1674-2818; 2049-3169
• DOI: 10.4248/ijos.08020

Aim To detect the expression of HSP25 in rat dental follicles both in vivo and vitro, and explore the underlying mechanism of HSP25 on the proliferation and differentiation of rat dental follicle cells （DFCs）. Methodology Immunohistochemistry was performed to detect the expression of HSP25 in mandibles of postnatal rats on days 1, 3, 5, 7, 9 and 11 in vivo. In vitro, the expression of HSP25 in DFCs was detected by an indirect immunofluorescence assay. Thiazolyl blue tetrazolium bromide （MTT） assay, flow cytometry and alkaline phosphatase （ALP） assay were used to identify the time-course effect mediated by different concentrations of recombinant murine HSP25 of 0, 1, 10, 50 and 100 ng/mL on rat DFCs. Results Expression of HSP25 was not detected in dental follicles of the rats until day 5 after birth, but became up-regulated in a time-dependent manner till day 11. HSP25 was detected in the cytoplasm of cultured rat DFCs. No significant difference could be observed in the proliferation of DFCs after stimulation with different concentrations of HSP25 on days 1, 2 and 3 （P〉0.05）. HSP25 at concentrations of 50 ng/mL and 100 ng/mL up-regulated the ALP activity of DFCs on day 9 （P〈0.05）. Conclusion HSP25-immunoreactivity increased chronologically during the development of dental follicles. The protein had no significant effect on cell proliferation but may play a role in cementoblast/osteoblast differentiation of DFCs.