Antimicrobial photodynamic inactivation as an alternative approach to inhibit the growth of Cronobacter sakazakii by fine-tuning the activity of CpxRA two-component system
is an opportunistic foodborne pathogen primarily found in powdered infant formula (PIF). To date, it remains challenging to control the growth of this ubiquitous bacterium. Herein, antimicrobial photodynamic inactivation (aPDI) was first employed to inactivate . Through 460 nm light irradiation coup...
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Published in | Frontiers in microbiology Vol. 13; p. 1063425 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
17.01.2023
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Subjects | |
Online Access | Get full text |
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Summary: | is an opportunistic foodborne pathogen primarily found in powdered infant formula (PIF). To date, it remains challenging to control the growth of this ubiquitous bacterium. Herein, antimicrobial photodynamic inactivation (aPDI) was first employed to inactivate
. Through 460 nm light irradiation coupled with hypocrellin B, the survival rate of
was diminished by 3~4 log. The photokilling effect was mediated by the attenuated membrane integrity, as evidenced by PI staining. Besides, scanning electron microscopy showed the deformed and aggregated cell cluster, and intracellular ROS was augmented by 2~3 folds when light doses increase. In addition to planktonic cells, the biofilm formation of
was also affected, showing an OD
decline from 0.85 to 0.25. In terms of molecular aspects, a two-component system called CpxRA, along with their target genes, was deregulated during illumination. Using the knock-out strain of ΔCpxA, the bacterial viability was reduced by 2 log under aPDI, a wider gap than the wildtype strain. Based on the promoted expression of
and
, aPDI is likely to play its part through attenuating the function of CpxRA-OmpC pathway. Finally, the aPDI system was applied to PIF, and
was inactivated under various desiccated or heated storage conditions. Collectively, aPDI serves as an alternative approach to decontaminate
, providing a new strategy to reduce the health risks caused by this prevalent foodborne pathogen. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology Reviewed by: Arunachalam Muthaiyan, University of New Mexico Gallup, United States; Michael R. Hamblin, University of Johannesburg, South Africa Edited by: Hedayat Hosseini, Shahid Beheshti University of Medical Sciences, Iran |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2022.1063425 |