Pharmacokinetics, bioavailability, and excretion of ponazuril in piglets

• Published in: Frontiers in veterinary science Vol. 9; p. 1054417
• Main Authors: Wang, Wenxiang, Ma, Yuqiao, Zhang, Yunxiao, Nie, Jingjing, Hu, Daxing, Yang, Weicong, Shen, Yue, Cui, Xinglong, Ding, Huanzhong, Li, Li, Huang, Xianhui
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 07.12.2022
• Subjects: bioavailability; excretion; NLME; nonlinear pharmacokinetics; piglets; ponazuril; Veterinary Science; NLME; piglets; excretion; ponazuril; bioavailability; nonlinear pharmacokinetics
• ISSN: 2297-1769; 2297-1769
• DOI: 10.3389/fvets.2022.1054417

Ponazuril is a triazine anticoccidial drug which is the main metabolite of toltrazuril in animals, it has excellent activity against many protozoa, including , and has broad application prospects in the control of swine coccidiosis. To evaluate the pharmacokinetic and excretion characteristics of ponazuril, 12 healthy piglets aged 10-14 days were divided into 2 groups for pharmacokinetic studies, which were given 20 mg/kg body weight ponazuril orally and intravenously, respectively. And 6 other piglets were housed individually in metabolic cages and given the same oral dose of ponazuril. After administration, the concentration of ponazuril in plasma, fecal, and urine samples collected was determined using high-performance liquid chromatography (HPLC). The plasma concentration profiles of ponazuril obtained after intravenous and oral administration were analyzed simultaneously by the nonlinear mixed-effects (NLME) model. Following the results, the pharmacokinetics of ponazuril exhibited a Michaelis-Menten elimination with Michaelis-Menten constant K and maximum metabolic rate V of 10.8 μg/mL and 0.083 mg/kg/h. The apparent volume of distribution was calculated to be 735 mL/kg, and the final estimated oral bioavailability was 81%. Besides, cumulatively 86.42 ± 2.96% of ponazuril was recovered from feces and 0.31% ± 0.08% from urine during 0-1,020 h after oral administration. These findings indicated a good oral absorption of ponazuril in piglets with nonlinear disposition and slow excretion largely feces, implying sustained drug concentration and long-lasting anticoccidial effects.