Simultaneous quantification of the viral antigens hemagglutinin and neuraminidase in influenza vaccines by LC–MSE

• Published in: Vaccine Vol. 30; no. 32; pp. 4762 - 4770
• Main Authors: Creskey, Marybeth C, Li, Changgui, Wang, Junzhi, Girard, Michel, Lorbetskie, Barry, Gravel, Caroline, Farnsworth, Aaron, Li, Xuguang, Smith, Daryl G.S, Cyr, Terry D
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 06.07.2012; Elsevier Limited
• Subjects: Acquisitions & mergers; Allergy and Immunology; Animals; antibodies; Antigens; Chickens; Chromatography, Liquid; Data processing; Egg Proteins - analysis; eggs; enzyme activity; Hemagglutinin; Hemagglutinin Glycoproteins, Influenza Virus - analysis; hemagglutinins; HPLC; immune response; Infections; Influenza; Influenza vaccine; Influenza Vaccines - analysis; Influenza Vaccines - chemistry; Influenza Vaccines - standards; Ligands; Manufacturers; Manufacturing; Mass Spectrometry; Methods; Neuraminidase; Neuraminidase - analysis; ovalbumin; pandemic; Peptides; protein content; Proteins; quality control; Quantification; Reference Standards; Reproducibility of Results; sialidase; Signal transduction; Software; vaccine development; Vaccines; viral antigens; Quantification; Hemagglutinin; Influenza vaccine; Mass spectrometry; HPLC; Neuraminidase
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2012.05.036

Highlights ► A new method has been adapted for analysis of influenza vaccines. ► A new method was optimized for optimal reproducibility and ruggedness. ► A new method was verified with NIST Reference Standard and model proteins. ► A new method was applied to monovalent (pandemic) and trivalent (annual) vaccines. ► Results were compared with standard methods (HPLC, Western blot, SRID). ► Results provide quantitation of HA, NA and other proteins.