Genes That Are Uniquely Stress Regulated in Salt Overly Sensitive (sos) Mutants

• Published in: Plant physiology (Bethesda) Vol. 126; no. 1; pp. 363 - 375
• Main Authors: Gong, Zhizhong, Koiwa, Hisashi, Mary Ann Cushman, Anamika Ray, Davi Bufford, Shin Kore-eda, Matsumoto, Tracie K., Zhu, Jianhua, Cushman, John C., Bressan, Ray A., Hasegawa, Paul M.
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.05.2001; American Society of Plant Biologists
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - physiology; Biological and medical sciences; CCR1 gene; Complementary DNA; DNA, Plant; Environmental Stress and Adaptation; Expressed Sequence Tags; Fundamental and applied biological sciences. Psychology; Gene expression regulation; Gene Expression Regulation, Plant - drug effects; Genes; Genes, Plant; Messenger RNA; Molecular Sequence Data; Mutation; Open Reading Frames; Plant physiology and development; Plants; Polymerase chain reaction; Salt tolerance; Salts; SAMT gene; Seedlings; Sodium chloride; Sodium Chloride - pharmacology; SOS1 gene; STZ gene; Table salt; VSP2 gene; Water and solutes. Absorption, translocation and permeability; Yeasts; Salt resistance; Ionic regulation; Genetic variant; Homeostasis; Osmoregulation; Identification; Gene expression; Stress; Salinity; Arabidopsis thaliana; Regulation(control); Gene; Cruciferae; Dicotyledones; Angiospermae; Spermatophyta; Experimental plant; Adaptation
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.126.1.363

Repetitive rounds of differential subtraction screening, followed by nucleotide sequence determination and northern-blot analysis, identified 84 salt-regulated (160 mM NaCl for 4 h) genes in Arabidopsis wild-type (Col-0 gl1) seedlings. Probes corresponding to these 84 genes and ACP1, RD22BP1, MYB2, STZ, and PAL were included in an analysis of salt responsive gene expression profiles in gl1 and the salt-hypersensitive mutant sos3. Six of 89 genes were expressed differentially in wild-type and sos3 seedlings; steady-state mRNA abundance of five genes (AD06C08/unknown, AD05E05/vegetative storage protein 2 [VSP2], AD05B11/S-adenosyl-L-Met:salicylic acid carboxyl methyltransferase [SAMT], AD03D05/cold regulated 6.6/inducible2 [COR6.6/KIN2], and salt tolerance zinc finger [STZ]) was induced and the abundance of one gene (AD05C10/circadian rhythm-RNA binding1 [CCR1]) was reduced in wild-type plants after salt treatment. The expression of CCR1, SAMT, COR6.6/KIN2, and STZ was higher in sos3 than in wild type, and VSP2 and AD06C08/unknown was lower in the mutant. Salt-induced expression of VSP2 in sos1 was similar to wild type, and AD06C08/unknown, CCR1, SAMT, COR6.6/KIN2, and STZ were similar to sos3. VSP2 is regulated presumably by SOS2/3 independent of SOS1, whereas the expression of the others is SOS1 dependent. AD06C08/unknown and VSP2 are postulated to be effectors of salt tolerance whereas CCR1, SAMT, COR6.6/KIN2, and STZ are determinants that must be negatively regulated during salt adaptation. The pivotal function of the SOS signal pathway to mediate ion homeostasis and salt tolerance implicates AD06C08/unknown, VSP2, SAMT, 6.6/KIN2, STZ, and CCR1 as determinates that are involved in salt adaptation.