Rapid determination on sperm cell concentration in bovine semen by flow cytometry

A flow cytometric technique is described for determining sperm concentration in fresh or extended semen with improved accuracy, precision, repeatability, ease of conduct, and rapidity. The technique is designed to measure the ratio of a known number of fluorescent beads admixed with sperm stained wi...

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Bibliographic Details
Published inJournal of dairy science Vol. 76; no. 1; pp. 86 - 94
Main Authors Evenson, D.P. (South Dakota State University, Brookings), Parks, J.E, Kaproth, M.T, Jost, L.K
Format Journal Article
LanguageEnglish
Published Savoy, IL Am Dairy Sci Assoc 01.01.1993
American Dairy Science Association
Subjects
Acridine Orange
ADN
Animals
Biological and medical sciences
Cattle
COLORANT
COLORANTES
CONSERVACION DEL SEMEN
CONSERVATION DU SPERME
ESPERMATOZOO
Flow Cytometry
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
Male
Mammalian male genital system
Microspheres
Morphology. Physiology
Propidium
SEMEN
Semen - cytology
Sperm Count
SPERMATOZOIDE
SPERME
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
Vertebrates: reproduction
Flow cytometry
Vertebrata
Spermatozoa
Rapid technique
Mammalia
Bovine
Semen
Quality
Fluorescence
Artiodactyla
Concentration
Ungulata
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Summary:A flow cytometric technique is described for determining sperm concentration in fresh or extended semen with improved accuracy, precision, repeatability, ease of conduct, and rapidity. The technique is designed to measure the ratio of a known number of fluorescent beads admixed with sperm stained with either acridine orange or propidium iodide. A significant advantage of the technique is the distinct resolution between sperm and other particles (e.g., somatic cells, fat droplets, and bacteria in the semen or extender) that interfere in other counting protocols. Field testing of this protocol over the past 3 yr has demonstrated its superiority over the Coulter counter, hemacytometer, and spectrophotometer for accuracy in counting sperm in extended semen and the accuracy of counting sperm in straws based on preextension spectrophotometric determination of sperm concentration. Sperm chromatin quality can be determined simultaneously with this sperm counting procedure. This approach to counting sperm provides an excellent procedure for quality control of sperm numbers in processed semen
Bibliography:9334177
L53
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(93)77326-9