Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania

• Published in: Frontiers in pharmacology Vol. 14; p. 1115117
• Main Authors: Babotă, Mihai, Frumuzachi, Oleg, Nicolescu, Alexandru, Stojković, Dejan, Soković, Marina, Rocchetti, Gabriele, Zhang, Leilei, Lucini, Luigi, Crișan, Gianina, Mocan, Andrei, Voștinaru, Oliviu
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 17.02.2023
• Subjects: antimicrobials; diuretic; Pharmacology; polyphenols; Thymus comosus Heuff. ex. Griseb; wild thyme; polyphenols; Thymus comosus Heuff. ex. Griseb; antimicrobials; diuretic; wild thyme
• ISSN: 1663-9812; 1663-9812
• DOI: 10.3389/fphar.2023.1115117

Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product , cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the diuretic effect and antimicrobial properties of three herbal preparations (infusion-TCI, tincture-TCT and an hydroethanolic extract prepared through an optimized ultrasound-assisted method-OpTC) obtained from the aerial parts of Heuff ex. Griseb, also evaluating their comprehensive phenolic profile. diuretic effect was tested using Wistar rats treated orally with each herbal preparation (125 and 250 mg/kg dispersed in 25 ml/kg isotonic saline solution) and quantified based on cumulative urine output (ml), diuretic action and diuretic activity. Additionally, sodium and potassium excretion were monitored using a potentiometric method with selective electrodes. antibacterial and antifungal activities were assessed using -iodonitrotetrazolium chloride assay against six bacterial strains and six fungal strains by monitoring minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs). Finally, phenolic profile of the aforementioned herbal extracts was evaluated using an ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) method to check the impact of the different preparations on the most abundant and significant compounds. All the extracts exerted a mild diuretic action, TCT and OpTC inducing the most intense diuretic effect. Both herbal preparations produced a statistically significant, dose-dependent and gradual increase of the urine output, the effect being more intense at 24 h (6.63-7.13 ml/24 h). Potentiometric evaluation of urine samples collected from treated rats revealed a clear and mild natriuretic and kaliuretic effect after the administration. In terms of antimicrobial activity, (MIC-0.38 mg/ml), (MIC-0.75 mg/ml)), and var. (MIC-0.19 mg/ml) showed the greater sensitivity to the tested extracts, respectively. UHPLC-HRMS screening showed that the bioactive potential of herbal preparations was likely related to the higher amounts of phenolic acids (including rosmarinic acid), flavonoids (mainly flavones and derivatives) and other phenolics (such as different isomers of salvianolic acids) in their composition. The obtained results support the ethnopharmacological evidence regarding the mild diuretic and antibacterial potentials of the endemic wild thyme , this study being the first one that assessed the aforementioned bioactivities for this species.