Simple method for the routine determination of betaine and N,N-dimethylglycine in blood and urine

A simple and convenient method using commercially available derivatization reagents is described for the measurement of betaine and N,N-dimethylglycine (DMG) in blood and urine. Precolumn derivatization of plasma or urine is performed directly in acetonitrile without extraction with p-bromophenacyl...

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Bibliographic Details
Published inClinical chemistry (Baltimore, Md.) Vol. 44; no. 9; pp. 1937 - 1941
Main Authors Laryea, Maurice D, Steinhagen, Folkert, Pawliczek, Sandra, Wendel, Udo
Format Journal Article Conference Proceeding
LanguageEnglish
Published Washington, DC Am Assoc Clin Chem 01.09.1998
American Association for Clinical Chemistry
Subjects
Adult
Analysis
Betaine - blood
Betaine - urine
Biological and medical sciences
Child
Child, Preschool
Chromatography, High Pressure Liquid
General pharmacology
Humans
Infant
Medical sciences
Middle Aged
Pharmacology. Drug treatments
Reference Values
Sarcosine - analogs & derivatives
Sarcosine - blood
Sarcosine - urine
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Human
Urine
Biological fluid
Ultraviolet detector
Betaine
Metabolite
HPLC chromatography
Routine analysis
Isocratic condition
Quantitative analysis
Blood plasma
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Summary:A simple and convenient method using commercially available derivatization reagents is described for the measurement of betaine and N,N-dimethylglycine (DMG) in blood and urine. Precolumn derivatization of plasma or urine is performed directly in acetonitrile without extraction with p-bromophenacyl bromide and crown ether as catalyst. The p-bromophenacyl ester derivatives are then separated by high-performance liquid chromatography, using an isocratic system of acetonitrile and water containing choline. Effluent was monitored at 254 nm. The limit of detection was 5 micromol/L for betaine and 2 micromol/L for DMG. Analytical recovery was >97% for both analytes. Total and within-day CVs were 2.0-4.4% and 0.9-2.2% for DMG. For betaine, the total and within-day CVs were 1.3-5.3% and 0.4-3.8%, respectively. The method is precise and cost-effective and has been used successfully to determine the concentrations of DMG and betaine in human plasma and urine.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/44.9.1937