Efficacy of synergistic activity of seed oils from Carthamus tinctorius (Safflower) and Nasturtium officinale (Watercress) on the lethality of the cattle tick Hyalomma scupense (Acari: Ixodidae)

• Published in: Open veterinary journal (Tripoli, Libya) Vol. 12; no. 1; pp. 80 - 90
• Main Authors: Alimi, Dhouha, Hajri, Azhar, Jallouli, Selim, Sebai, Hichem
• Format: Journal Article
• Language: English
• Published: Libya Faculty of Veterinary Medicine 01.01.2022; Tripoli University
• Subjects: acaracidal activity; Animals; carthamus tinctorius; Carthamus tinctorius - chemistry; Female; h. scupense; Ixodidae; Linoleic Acid; Nasturtium; nasturtium officinale; Original Research; Palmitic Acid; Plant Oils - chemistry; Plant Oils - pharmacology; Rhipicephalus; synergistic; Acaracidal activity; Carthamus tinctorius; synergistic; H. scupense; Nasturtium officinale
• ISSN: 2218-6050; 2226-4485; 2218-6050
• DOI: 10.5455/OVJ.2022.v12.i1.10

Ticks and tick-borne diseases are a severe economic and public-health problem for cattle producers. The emergence of acaricide resistance to synthetic chemical treatments has prompted interest in developing alternative tick control methods. The main objective of the current research was to identify the chemical structure of and seed oils and to assess their anti-tick properties against ticks both alone and in combination (1:1). Analytical methods were used to analyze the chemical components. For assays, adults of were immersed in and seed oils at 100, 125, 200, and 300 mg/ml concentrations; for 5 minutes. Larvae of were dipped in 25, 50, 100, 125, 200, and 300 mg/ml doses of seed oils; the mortality percentage was determined after 24 hours. The seed oil safflower was mainly composed of linoleic acid (84.48%), followed by palmitic acid (6.54%) and stearic acid (3.77%). Meanwhile, watercress seed oil was mainly composed of linolenic acid (50.78%), gondoic acid (13.57%), linoleic acid (10.58%), palmitic acid (8.02%), and erucidic acid (6.62%). The Adults Immersion Test showed the sensitivity of ticks to and seed oil: seed oil caused (95%) mortality of at 300 mg/ml, while seed oil induced (88.68%) mortality at the same concentration. At a 200 mg/ml concentration, and oil combined caused 100% mortality. Tested oils showed larvicidal efficacy LC50 values for and seed oils were 84.16 and 61.78 mg/ml, respectively, in 24 hours. LC50 value of oils association (50% 50% ) was 47.96 mg/ml.The mixture of seed oils from two plants tested against larvae and adult females at a 1:1 ratio showed synergistic interaction. Seed oils tested alone, and the mixture could be used as an alternative solution in the fight against ticks.