Pyramiding of two bacterial blight resistance and a semidwarfing gene in Type 3 Basmati using marker-assisted selection

• Published in: Euphytica Vol. 178; no. 1; pp. 111 - 126
• Main Authors: Rajpurohit, Deepak, Kumar, Rahul, Kumar, Mankesh, Paul, Priyanka, Awasthi, Anjali, Osman Basha, P, Puri, Anju, Jhang, Tripta, Singh, Kuldeep, Dhaliwal, Harcharan Singh
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.03.2011; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; Bacteria; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Crop diseases; Disease susceptibility; Fundamental and applied biological sciences. Psychology; Genes; Genetic markers; Genetics and breeding of economic plants; Genotype & phenotype; Life Sciences; Nitrogen fertilizers; Oryza sativa; Pest resistance; Plant genetics; Plant Genetics and Genomics; Plant pathogens; Plant Pathology; Plant Physiology; Plant resistance; Plant Sciences; Rice; Selective breeding; Varietal selection. Specialized plant breeding, plant breeding aims; Basmati rice; Resistance gene; pv; Pyramiding; Semidwarf; MAS; Bacterial blight; Background selection; Pseudomonadales; Monocotyledones; Plant pathogen; Marker assisted selection; Xanthomonas oryzae; Genetic determinism; Cereal crop; Oryza sativa; Microorganism resistance; Gramineae; Angiospermae; Pseudomonadaceae; Bacteria; Gene pyramiding; Plant height; Genetic improvement; Spermatophyta; Xanthomonas oryzae pv. oryzae
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-010-0279-8

A traditional Type 3 Basmati rice cultivar grown in India is tall and lodges even under low nitrogen fertilizer dose. In addition to lodging, it is highly susceptible to several diseases and pests including bacterial blight (BB). BB resistance genes (Xa21 and xa13) and a semidwarfing gene (sd-1) were pyramided in Type 3 Basmati from a rice cultivar PR106-P2 using marker-assisted selection (MAS). Foreground selection for BB resistance genes, Xa21 and xa13 and reduced plant height gene, sd-1 was carried on the basis of linked molecular markers pTA248, RG136 and ‘h', respectively. The BC₂F₃ progenies with both the BB resistance genes were highly resistant with lower lesion length than either of the genes individually. Background profiling of the selected 16 BC₂F₃ progenies was done using 95 anchored SSR and 12 ISSR markers. Among the selected 16 BC₂F₃ progenies, 38-5-2 and 38-5-36 closely clustered along with the recipient parent Type 3 Basmati showing above 85% genetic similarity with the same. Further selection was continued till F₅ generation for higher recovery for Type 3 Basmati characteristics. The desirable alleles of intermediate amylose content (wx) and aroma (fgr) loci of Type 3 Basmati were also tracked using the linked SSR markers. The BC₂F₅ pyramid lines T3-4, T3-5, T3-6 and T3-7 homozygous for the three target genes Xa21, xa13 and sd-1 from the donor parent with wx and fgr alleles of Type 3 Basmati had excellent cooking quality and strong aroma.